Qstar elite system

Optical rotations were measured on a JASCO DIP-1000 polarimeter, with a Na (589 nm) lamp and filter. IR spectra were measured on a FTIR Bruker Vector 22 spectrometer. ¹H, ¹³C, and 2D NMR spectra were recorded on a Bruker Avance 500 spectrometer at 500 and 125 MHz, respectively, using CDCl₃. Low resolution electrospray mass spectrometry (LRESIMS) and high resolution electrospray mass spectrometry (HRESIMS) experiments were performed on the Applied Biosystems QSTAR Elite system. LREIMS and HREIMS were performed on the Mass Spectrometer Thermo MAT95XP. HPLC separations were performed on the Agilent 1100 liquid chromatography system equipped with a solvent degasser, quaternary pump, and diode array detector (Agilent Technologies, Waldbronn, Germany) using a semipreparative reversed phase column Luna C18, 5 µ, 100 Å, 250 × 10 mm. Precoated silica gel plates (Merck, Kieselgel 60 F254, 0.25 mm) were used for TLC analysis, and the spots were visualized under a UV light (254 nm) or by heating the plate pretreated with H₂SO₄/H₂O/AcOH (1:4:20).

Optical rotations were measured on a JASCO DIP-1000 polarimeter, with a Na (589 nm) lamp and filter. IR spectra were measured on a FTIR Bruker Vector 22 spectrometer. ¹H, ¹³C, and 2D NMR spectra were recorded on a Bruker Avance 500 spectrometer at 500 and 125 MHz, respectively, using CDCl₃. Low resolution electrospray mass spectrometry (LRESIMS) and high-resolution electrospray mass spectrometry (HRESIMS) experiments were performed on the Applied Biosystems QSTAR Elite system. The chemical shifts were given in δ (ppm) and coupling constants in Hz. HPLC separations were performed on the Agilent 1100 liquid chromatography system equipped with a solvent degasser, quaternary pump, and diode array detector (Agilent Technologies, Waldbronn, Germany) using a semipreparative normal phase column Nova-pak, silica 6 µm 60 Å, 300 × 7.8 mm (Waters). Precoated silica gel plates (Merck, Kieselgel 60 F254, 0.25 mm) were used for TLC analysis, and the spots were visualized under a UV light (254 nm) or by heating the plate pre-treated with H₂SO₄/H₂O/AcOH (1:4:20)^{25 (link)}.

Optical rotations were measured on a JASCO DIP-1000 polarimeter, with an Na (589 nm) lamp and filter. ¹H, ¹³C, and 2D NMR spectra were recorded on a Bruker spectrometer of 950 MHz, equipped with a 5 mm Cryo Probe (NEO console); a Bruker spectrometer (800 MHz for ¹H and 200 MHz for ¹³C) equipped with a 5 mm Cryo Probe and an NEO console; a Bruker (800 MHz for ¹H and 200 MHz for ¹³C) spectrometer equipped with a 3 mm Cryo Probe and a NEO console; a Bruker spectrometer (700 MHz for ¹H and 175 MHz for ¹³C) equipped with a 5 mm Cryo Probe and an Avance III console; and a Bruker Avance 500 spectrometer (500 MHz for ¹H and 125 MHz for ¹³C) equipped with a 5 mm Cryo Probe, using DMSO-d₆ and CDCl₃ as solvents. Chemical shifts are reported in δ scale relative to DMSO-d₆ (δ 2.50 ppm for ¹H NMR, δ 39.51 ppm for ¹³C NMR) and CDCl₃ (δ 7.26 ppm for ¹H NMR, δ 77.0 ppm for ¹³C NMR).
HRESIMS experiments were performed on the Applied Biosystems QSTAR Elite system or a Thermo MAT95XP spectrometer. HPLC separations were performed on the Agilent 1100 liquid chromatography system equipped with a solvent degasser, quaternary pump, and diode array detector (Agilent Technologies, Waldbronn, Germany) using a semipreparative reversed phase column Luna C18 (5 μ, 100 Å, 250 × 10 mm).