Rat high range insulin elisa

P-Rex1 antibody was from R&D Systems (Minneapolis, MN, USA). Antisera directed against RhoG was from Santa Cruz Biotechnology (CA, USA). E-Cadherin, GAPDH and HRP-conjugated secondary antibodies were from Cell Signaling (Danvers, MA, USA). Rac1 antibody was from EMD Millipore (Burlington, MA, USA). Rat high range insulin ELISA was from ALPCO (Salem, NH, USA). Target sequence for the ON-TARGETplus Non-targeting siRNA#1 (Catalog Item- D-001810–01-20) is: UGGUUUACAUGUCGACUAA. The target sequences for the ON-TARGETplus Rat Prex1(311647) siRNA-SMARTpool (Catalog Item L-093719–02-0010 that has 4 sequences that it targets) are: GCAUGGAGCGCGACGCAUA, CAACAACAACGGCGAGUAU, UCCUGAAAGUCAACGGCAA and CCAUCAACGCCCUGGACGA. The above on-target P-Rex1siRNA SMARTpool and non-targeting control siRNA (Con-si), as well as DharmaFect1, were from Dharmacon (Lafayette, CO, USA). Antibody for β-actin and all other reagents used in the current studies were from Sigma Aldrich (St. Louis, MO, USA). Mem-PER Plus Membrane Extraction Kit was from Thermo Fisher Scientific (Waltham, MA, USA). The pull down assay kit used for the Rac1 activation was from Cytoskeleton (Denver, CO, USA).