Goat anti mouse igg h l alexa488

Manufactured by Thermo Fisher Scientific

Sourced in United States

Goat-anti-mouse IgG(H+L)-Alexa488 is a secondary antibody conjugated with Alexa Fluor 488 dye. It is designed to detect and bind to mouse immunoglobulin (IgG) heavy and light chains.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Alexa488-conjugated goat anti-mouse IgG (H+L) antibody Goat anti-mouse IgG (H+L) H + L IgG Mouse anti-IgG

7 protocols using goat anti mouse igg h l alexa488

HCV Viral Spread Assay in Huh7.5 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Huh7.5 cells were plated at 4x10⁵ per well in 6-well plates 24 hours prior to transfection at which point they were ~70% confluent. Plasmids were linearized by XbaI treatment (New England BioLabs). RNA was generated by T7-mediated in-vitro transcription and was transfected into Huh7.5 cells using lipofectamine 2000 (Invitrogen). 6 hours post transfection, Huh7.5 cells were trypsinized and reseeded into four wells of 24-well plates at a cell density of 8x10⁴ (for the 4 time-points of the assay) along with plating in 6-well chamber slides for assessing percent infected cells at the four time-points, using the primary mouse anti-HCV NS5A 9E10 antibody and the secondary antibody Alexa488 goat anti-mouse IgG (H+L) (Invitrogen) as described [59 (link)] and Hoechst 33342 (Molecular Probes) counterstain for nuclei. Viral spread was monitored every 24 hours along with harvesting of virus supernatant up to 96 hours post transfection. Supernatants collected during experiments were sterile filtered and stored at −80°C. The virus titers were determined as described previously [79 (link), 80 (link)].

Velázquez-Moctezuma R., Law M., Bukh J, & Prentoe J. (2017). Applying antibody-sensitive hypervariable region 1-deleted hepatitis C virus to the study of escape pathways of neutralizing human monoclonal antibody AR5A. PLoS Pathogens, 13(2), e1006214.

+ Open protocol

+ Expand

Antibody Panel for Neural Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies used for immunostaining and immunoblot included rabbit anti-MAP2 (CST, #4542, 1:1000), mouse anti-tubulin β III (Covance, MMS-435P, 1:5000), rabbit anti-tubulin β III (Covance, PRB-435P-100, 1:1000), chicken anti-beta-tubulin 3 (Aves Labs, AB_2313564, 1:1000), rabbit anti-p62/SQSTM1 (Abcam, ab109012, 1:1000), mouse anti-p62/SQSTM1 (CST, #88588, 1:1000), rabbit anti-STAT3 (CST, #4904, 1:1000), rabbit anti-GAPDH (Santa Cruz, sc-32233, 1:5000), rabbit anti-DARPP-32(19A3) (CST, #2306, 1:200), rat anti-CTIP2 (Abcam, ab18465, 1:500), rabbit anti-DLX1 (Millipore, AB5724, 1:100), rabbit anti-DLX2 (Abcam, ab135620, 1:100), and rabbit anti-MYT1L (Proteintech, 25234-1-AP, 1:500) antibodies. The secondary antibodies for immunostaining included Alexa-488 Goat anti-mouse IgG (H+L) (Invitrogen, A-11029, 1:2000), Alexa-488 Goat anti-rabbit IgG (H+L) (Invitrogen, A-11034, 1:2000), Alexa-568 Goat anti-mouse IgG (H+L) (Invitrogen, A-11031, 1:2000), Alexa-568 Goat anti-rabbit IgG (H+L) (Invitrogen, A-11016, 1:2000), Alexa-594 Goat anti-mouse IgG (H+L) (Invitrogen, A-11032, 1:2000), Alexa-594 Goat anti-rabbit IgG (H+L) (Invitrogen, A-11012, 1:2000), Alexa-647 Goat anti-chicken IgY (Invitrogen, A-21449, 1:2000), and Alexa-594 Goat anti-rat IgG (H+L) (Invitrogen, A-11007, 1:2000).

Oh Y.M., Lee S.W., Kim W.K., Chen S., Church V.A., Cates K., Li T., Zhang B., Dolle R.E., Dahiya S., Pak S.C., Silverman G.A., Perlmutter D.H, & Yoo A.S. (2022). Age-related Huntington’s disease progression modeled in directly reprogrammed patient-derived striatal neurons highlights impaired autophagy. Nature neuroscience, 25(11), 1420-1433.

+ Open protocol

+ Expand

Flow Cytometry Analysis of Differentiation and Maturation Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Differentiation and maturation marker expression was measured using flow cytometry. For differentiation markers the following antibodies were used: mouse-anti human CD206 (MMR; Clone 19.2) and mouse anti-human CD209 (DC-SIGN; Clone DCN46), both from BD Pharmingen; mouse anti-human CD14 (clone RM052; Immunotech), mouse anti-human β₂ integrins (clone L19; from hybridoma) and rat anti-human β₁ integrins (clone AIIB2; from hybridoma), and mouse anti-human CD68 (clone KP1; eBioscience). For maturation markers the following antibodies were used: mouse anti-human CCR7 (clone 150503, R&D Systems), mouse anti-human MHC-II (HLA-DR/DP; clone q5/13, from hybridoma), mouse anti-human CD83-FITC (clone HB15e) and mouse anti-human CD86-PE (clone 2331), both BD. Appropriate isotype controls were included in all measurements.
Goat-anti-mouse IgG(H+L)-Alexa488 or goat-anti-rat IgG(H+L)-Alexa488 secondary antibodies were used (Life Technologies). Cell viability was monitored with Fixable Viability Dye eFluor780 (eBioscience). Samples were measured on a CyAn ADP flow cytometer (Beckman Coulter). Analysis was performed with Flowjo Software (Treestar Inc) version 9.7.6. Cell surface marker geometric mean fluorescence intensities (gMFI) were obtained from the eFluor780-negative cell fraction and corrected with geometric mean fluorescence intensities measured for isotype controls.

Mennens S.F., Bolomini-Vittori M., Weiden J., Joosten B., Cambi A, & van den Dries K. (2017). Substrate stiffness influences phenotype and function of human antigen-presenting dendritic cells. Scientific Reports, 7, 17511.

+ Open protocol

+ Expand

Immunofluorescence Analysis of Skin Substitutes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Skin substitute biopsies from each condition were embedded in Tissue-Tek Optimal Cutting Temperature (O.C.T.) Compound (Sakura Finetek, Torrance, CA, USA) and frozen in liquid nitrogen. Indirect immunofluorescence staining was performed on five-micrometer-thick cryosections fixed in acetone. The primary antibodies used were: mouse monoclonal anti-Ki67 (IgG) (dilution 1:400, Abcam, Cambridge, MA, USA), chicken polyclonal anti-keratin 14 (IgY) (dilution 1:500, BioLegend, San Diego, CA, USA), mouse monoclonal anti-involucrin (IgG) (dilution 1:1600, Sigma) and rabbit polyclonal anti-loricrin (IgG) (dilution 1:1000, Cedarlane, Burlington, ON, Canada). The saturation of non-specific sites was performed simultaneously with the labeling of the primary antibodies, using animal serum from the same species as the secondary antibodies used. The secondary antibodies used were: goat anti-mouse IgG (H + L) Alexa 488 (dilution 1:500, Life Technologies, Eugene, OR, USA), donkey anti-chicken IgG (H + L) Alexa 488 (dilution 1:500, Jackson ImmunoResearch, West Grove, PA, USA) and donkey anti-rabbit IgG (H + L) Alexa 488 (dilution 1:500, Life Technologies). Cell nuclei were labeled after the secondary antibody with the mounting medium DAPI Fluoromount-G (SouthernBiotech, Birmingham, AL, USA).

Bouchard C., Grenier A., Cardinal S., Bélanger S., Voyer N, & Pouliot R. (2022). Antipsoriatic Potential of Quebecol and Its Derivatives. Pharmaceutics, 14(6), 1129.

+ Open protocol

+ Expand

Immunostaining of HEp-2 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEp-2 cells (ATCC) were split 1/3 in T175 on day −2 and seeded to glass coverslips at day −1 (100K/well, 24 well). Cells were fixed with 1%PFA for 20 min at RT. Cells were then blocked and permeabilized with block/perm buffer (2% FBS, 0.1% Tx-100 in PBS). After 30 min, cells were stained o/n in block/perm buffer with indicated antibodies at 10 ug/ml, 1 ug/ml or 0.1 ug/ml (C11 and 564 only). The next day, coverslips were washed 3 times and incubated for one hour with Goat anti Mouse IgG (H+L) Alexa488 (Life Technologies), phalloidin Alexa568 (Life Technologies) and DAPI. Coverslips were then washed 4 times and mounted on slides with Fluorogel as mounting medium. Detectors were set to no primary Ab control. Anti HA Ab 6649 was used as negative control. C11, the original hybridoma from which the 564 BCR was created was used as positive control in addition to re-cloned 564.

Degn S.E., van der Poel C.E., Firl D.J., Ayoglu B., Al Qureshah F.A., Bajic G., Mesin L., Reynaud C.A., Weill J.C., Utz P.J., Victora G.D, & Carroll M.C. (2017). Clonal Evolution of Autoreactive Germinal Centers. Cell, 170(5), 913-926.e19.

+ Open protocol

+ Expand

Immunoblotting and Histology Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunoblotting and histology, the following primary antibodies were used: anti-α-Actinin (sarcomeric) (A7811, Sigma Aldrich), anti-FBXO25 (gift from AG Bassermann (TUM MRI), anti-HSP90 α/β (Sc-13119, Santa Cruz). Secondary antibodies used: Goat anti-mouse IgG (H + L) Alexa 488 (A11029, Invitrogen), Goat anti-rabbit IgG (H + L) Alexa 488 (A11034, Invitrogen), anti-mouse HRP (7074S, Cell Signaling), anti-rabbit HRP (7076S, Cell Signaling). Penicillin, streptomycin, fetal bovine serum (FBS) and other reagents were obtained from Invitrogen Life Technologies (Carlsbad, CA) or Sigma-Aldrich (Louis, MO).

Fischer M., Jakab M., Hirt M.N., Werner T.R., Engelhardt S, & Sarikas A. (2023). Identification of hypertrophy-modulating Cullin-RING ubiquitin ligases in primary cardiomyocytes. Frontiers in Physiology, 14, 1134339.

+ Open protocol

+ Expand

Immunofluorescence Staining of Cellular Methylation Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

To perform immunofluorescence staining, the cells were fixed with 4% paraformaldehyde for 30 min and permeabilized with 0.1% Triton X-100 (Sigma) for 10 min. After washing three times with TBS-T, cells were blocked with 3% bovine serum albumin for 1 h. Cells were incubated with primary antibodies anti-DNMT1, anti-DNMT3A, anti-DNMT3B, anti-E-cadherin (1:200) diluted in 3% BSA overnight at 4 °C. Additionally, then the cells were incubated with secondary antibodies for 1 h. For secondary antibodies, Goat anti-Mouse IgG (H + L) Alexa 488 or Goat anti-Rabbit IgG (H + L) Alexa 555 (Invitrogen; 1:200) were used depending on the host of the primary antibodies. Nuclei were counterstained using 4’-6-diamidino-2-phenylindole (DAPI, Invitrogen). Fluorescence images were acquired using a confocal laser scanning microscope LSM700 (Zeiss, Oberkochen, Germany).

Park J.H., Shin J.M., Yang H.W, & Park I.H. (2022). DNMTs Are Involved in TGF-β1-Induced Epithelial–Mesenchymal Transitions in Airway Epithelial Cells. International Journal of Molecular Sciences, 23(6), 3003.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!