LIVE and DEAD® Viability/Cytotoxicity Kit (L3224 Thermo Fisher Scientific) was used. Staining was performed according to the manufacturer’s instructions.
Live and dead viability cytotoxicity kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The LIVE and DEAD® Viability/Cytotoxicity Kit is a fluorescence-based assay used to detect live and dead cells. The kit utilizes two fluorescent dyes: one that stains live cells and another that stains dead cells. This allows for the simultaneous quantification of live and dead cells in a sample.
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Lab products found in correlation
2 protocols using live and dead viability cytotoxicity kit
1
Viability and Cytotoxicity Assay
2
Cryopreservation of Melanoma Cell Lines
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Cryopreservation freezing solution was obtained from ZENOAQ RESOURCE CO., LTD (Fukushima, Japan). DAPI, Live and dead viability/cytotoxicity kit, and MTT assay kit were purchased from Thermo Fisher Scientific (Rockford, IL, USA). MS Collagen (Type 1 atelo-collagen from porcine skin) was obtained from MSBio, Inc. (Gyeonggi, Korea). Antibodies that recognize MITF (ab20663), Mel A (ab731), H2B (ab52599), S100 (ab4066), and tyrosinase (ab738) were purchased from Abcam (Cambridge, UK). Antibodies specific for actin (sc47778) were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). A375 melanoma cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). A375 cells were cultured in DMEM-high glucose with 10% FBS and 1% PS (penicillin-streptomycin).
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