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Anti pdk2

Manufactured by Proteintech
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Anti-PDK2 is a lab equipment product used for research purposes. It serves as an antibody that specifically targets the PDK2 (Pyruvate Dehydrogenase Kinase 2) protein, which plays a role in cellular metabolism. The core function of Anti-PDK2 is to enable the detection and study of PDK2 in various experimental settings.

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Lab products found in correlation

Bca kit, by Thermo Fisher Scientific (3 mentions) Daunomycin, by Merck Group (1 mentions) Uridine, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Glutamine, by Merck Group (1 mentions) 3 bromopyruvate, by Merck Group (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Coenzyme a, by Merck Group (1 mentions) Anti pdha1, by Proteintech (1 mentions) Anti trx2, by Proteintech (1 mentions) Anti pfkm, by Proteintech (1 mentions) Anti gr, by Proteintech (1 mentions) Anti idh2, by Proteintech (1 mentions) Anti fis1, by Cell Signaling Technology (1 mentions) Anti atp5b, by Proteintech (1 mentions) Anti hif 1α, by Proteintech (1 mentions) Anti catalase, by Proteintech (1 mentions) Anti mnsod, by Proteintech (1 mentions) Anti hk2, by Proteintech (1 mentions) Anti mfn1, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-TM (2 citations)

3 protocols using anti pdk2

1

Metabolic Modulation in Cancer Cells

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DCA, 3-bromopyruvate, sodium pyruvate, coenzyme A, glutamine, uridine and daunomycin were purchased from Sigma. Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were purchased from GIBCO. 14C-6-glucose was purchased from Moravek Biochemicals. Mouse monoclonal anti-P-glycoprotein was purchased from Santa Cruz Biotechnology and rabbit polyclonal anti-PDK2 was purchased from Protein Tech. 99mTc-MIBI (methoxyisobutylisonitrile) was purchased from Shanghai Hinko and 18 F-fluorodeoxyglucose was produced in our laboratory.
Zhou X., Chen R., Yu Z., Li R., Li J., Zhao X., Song S., Liu J, & Huang G. (2015). Dichloroacetate restores drug sensitivity in paclitaxel-resistant cells by inducing citric acid accumulation. Molecular Cancer, 14, 63.
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2

Mitochondrial Protein Expression Analysis

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Total cell extracts or nuclear extracts were separated by SDS-PAGE and transferred to PVDF membranes. The following antibodies were used for immunoblot analysis: anti-HK2, anti-PFKM, anti-LDHa1, anti-PDK2, anti-PDHa1, anti-ATP5B, anti-IDH2, anti-HIF1α, anti-MnSOD, anti-Cu/ZnSOD, anti-Catalase, anti-GR, anti-GRX1, anti-GPX1, anti-PRX3 and anti-TRX2 antibodies and secondary antibody were purchased from Proteintech. anti-MnSOD (acetyl K68) antibody was purchased from Abcam. Anti-β-actin, anti-ComplexV, anti-Lc3B, anti-MFN1, and anti-Fis1 antibodies were from Cell Signaling Technology. Protein expression is visualized on Tanon-5200 Chemi-luminescent Imaging System (Tanon Science Technology).
Li P., Zhang D., Shen L., Dong K., Wu M., Ou Z, & Shi D. (2016). Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells. Scientific Reports, 6, 22831.
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3

Western Blot Analysis of Signaling Proteins

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Whole cell lysates were extracted with RIPA buffer (Pierce, Thermo Scientific) containing protease inhibitor and PhosphStop cocktail (Roche). The total protein concentration was measured with a BCA kit according to the manufacturer’s instructions (Thermo Scientific). For Western blotting, the following antibodies were used: anti-HSC70 (catalog sc-7298) (Santa Cruz Biotechnology Inc.); anti-Pten (catalog 9559), anti-total AKT (catalog 4691), anti-phospho AKT (catalog 4060), anti-total S6 (catalog 2217), anti-phospho S6 (catalog 4858), anti-phospho GSK3b(catalog 5558), anti-mTOR (catalog 2972), anti-GFP (catalog 2956), anti-GST (catalog 2622), anti-Flag (catalog 8146), and anti-HA (catalog 3724) (Cell Signaling Technology); anti-Plek2 (catalog 11685-1-AP) and anti-PDK2 (catalog 15647-1-AP) (Proteintech); anti-Hsp72 (catalog PA5-34772) (Invitrogen); and HRP linked anti-GST antibody (catalog MA4-004-HRP) (Thermo Fisher Scientific). The Western blot results were further analyzed using Image Lab software (Bio-Rad).
Han X., Mei Y., Mishra R.K., Bi H., Jain A.D., Schiltz G.E., Zhao B., Sukhanova M., Wang P., Grigorescu A.A., Weber P.C., Piwinski J.J., Prado M.A., Paulo J.A., Stephens L., Anderson K.E., Abrams C.S., Yang J, & Ji P. (2023). Targeting pleckstrin-2/Akt signaling reduces proliferation in myeloproliferative neoplasm models. The Journal of Clinical Investigation, 133(6), e159638.
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