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Thermo ultimate uhplc system

Manufactured by Thermo Fisher Scientific
Sourced in Germany, United States

The Thermo Ultimate UHPLC system is a high-performance liquid chromatography instrument designed for efficient and precise separation and analysis of a wide range of chemical compounds. It features advanced technology for ultra-high-pressure liquid chromatography, enabling rapid and high-resolution separations.

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2 protocols using thermo ultimate uhplc system

1

Omics Analyses Using UHPLC-Q-TOF

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Omics analyses were performed on a Thermo Ultimate UHPLC system (Thermo Scientific, Bremen, Germany) coupled online to a TimsTOF Pro Quadrupole Time of Flight (Q-TOF) (Bruker Daltonics, Bremen, Germany) equipped with an Apollo II electrospray ionization (ESI) probe. Detailed instrument parameters are reported in Additional file 1: Section S.1.
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2

Carotenoid Extraction and Analysis in Arabidopsis

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All leaves from WT and transgenic A. thaliana plants were collected for carotenoid extraction in accordance with the method described by Ma et al. [70 (link)]. The β-carotene and lutein were separated using a Thermo UltiMate UHPLC System (Thermo, USA) with a Hedera ODS-2 C18 column (250 mm X 4.6 mm, 5 μm nominal particle size; Shimadzu, Japan), operated at a flow rate of 1 mL min− 1 at 30 °C with mobile phase containing methanol: acetonitrile (90:10, v/v). Detection was performed at 450 nm. Three biological replicates were used for carotenoids contents measurement.
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