Inveon acquisition workspace iaw

NOD/SCID (n = 3) mice bearing intracranial WK1 tumours were injected with IIIA4-NOTA-⁶⁴Cu (5–7 MBq) via the lateral tail vein. At 12 h post injection, mice were anaesthetised and maintained using 2% isoflurane in oxygen at a flow rate of 2 L/min and positioned on the scanning bed of an Inveon Preclinical PET/CT system (Siemens, Munich, Germany). A 30 min PET image was acquired followed by a CT for anatomical registration and attenuation correction. PET images were reconstructed using the OSEM-2D reconstruction algorithm in Inveon Acquisition Workspace (IAW, Siemens) correcting for isotope half-life, attenuation, and ⁶⁴Cu detector efficiency.

⁸⁹Zr radiolabeling was performed as described in the work of Zeglis and Lewis⁴⁰ and radiochemical yield and purity were determined by thin layer chromatography (TLC). Doses were administered if the radiochemical purity was more than 95%. PET–CT images were acquired 24 h post-FUS using an Inveon Preclinical PET-CT system (Siemens). Mice were anesthetized and maintained using 2% isoflurane in oxygen at a flow rate of 2 L/min and positioned in an in-house manufactured 4-mouse scanning bed. A 30 min PET image was acquired followed by a CT for attenuation correction and co-registration to the MRI data. The PET images were reconstructed using the OSEM-2D reconstruction algorithm in the Inveon Acquisition Workspace (IAW, Siemens) correcting for attenuation and ⁸⁹Zr detector efficiency.