Nanozoomer 2ht slide scanner

Manufactured by Hamamatsu Photonics

Sourced in Japan

The Nanozoomer 2HT slide scanner is a high-performance digital pathology slide scanner from Hamamatsu Photonics. It is designed to digitize glass slides for various applications, including tissue analysis and research. The device utilizes advanced optical and imaging technologies to capture high-resolution digital images of entire glass slides with a maximum scanning area of 15 mm x 15 mm.

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Lab products found in correlation

Axiocam hrc, by Zeiss (1 mentions) Axiocam mrc5, by Zeiss (1 mentions) Axiovision 4, by Zeiss (1 mentions) Axioplan brightfield microscope, by Zeiss (1 mentions) Axiovision software, by Zeiss (1 mentions) Ndp viewer software, by Hamamatsu Photonics (1 mentions)

Close spelling variants of this product

Nanozoomer 2HT NanoZoomer slide scanner Nanozoomer scanner Slide scanner NanoZoomer

4 protocols using nanozoomer 2ht slide scanner

Muscle Biopsy and Histological Analysis

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For P1, open deltoid muscle and radialis muscle biopsies were respectively obtained at 9 and 45 years of age. Conventional histological and histochemical techniques on 10 μm cryostat sections encompassed Haematoxylin and Eosin (H&E), Gomori Trichrome (GT), Nicotinamide adenosine dinucleotide–tetrazolium reductase (NADH-TR), Succinate dehydrogenase (SDH), and myosin adenosine triphosphatase (ATPase pH 9.4) reactions. Digital photographs were obtained with a Zeiss AxioCam HRc linked to a Zeiss Axioplan Bright Field Microscope and processed with the Axio Vision 4.4 software (Zeiss, Oberkochen, Germany).
For P2, open muscle biopsy of the quadriceps was obtained at 19 years of age. Digital photographs were obtained with a Zeiss AxioCam MRc5 linked to a Zeiss Imager Bright Field Microscope and processed with the AxioVision software (Zeiss).
Murine tibialis anterior muscles were sampled in isopentane cooled in liquid nitrogen, and transversal or longitudinal cryosections (8 μm) were fixed and stained with H&E, SDH, and GT. Images were acquired with the Hamamatsu NanoZoomer 2HT slide-scanner (Hamamatsu, Hamamatsu city, Japan).

Lornage X., Romero N.B., Grosgogeat C.A., Malfatti E., Donkervoort S., Marchetti M.M., Neuhaus S.B., Foley A.R., Labasse C., Schneider R., Carlier R.Y., Chao K.R., Medne L., Deleuze J.F., Orlikowski D., Bönnemann C.G., Gupta V.A., Fardeau M., Böhm J, & Laporte J. (2019). ACTN2 mutations cause “Multiple structured Core Disease” (MsCD). Acta neuropathologica, 137(3), 501-519.

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Histological Analysis of TA Muscle

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Transversal TA muscle cryosections of 8 μm were fixed and stained with H&E, NADH-TR, and SDH for histological analysis. Images were acquired using the Hamamatsu Nano Zoomer 2HT Slide Scanner. Fiber sizes were measured using Fiji software and fibers with abnormal SDH staining and nuclei position were counted using Cell Counter Plugin in Fiji software.

Lionello V.M., Kretz C., Edelweiss E., Crucifix C., Gómez-Oca R., Messaddeq N., Buono S., Koebel P., Massana Muñoz X., Diedhiou N., Cowling B.S., Bitoun M, & Laporte J. (2022). BIN1 modulation in vivo rescues dynamin-related myopathy. Proceedings of the National Academy of Sciences of the United States of America, 119(9), e2109576119.

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Histological Analysis of Muscle, Spleen, and Skin

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TA muscles were frozen in liquid nitrogen-cooled isopentane and transverse 8 µm sections were stained with hematoxylin and eosin (H&E), and the Cellpose algorithm [63 (link)] was used to segment and delineate the individual myofibers. The MinFeret diameter was calculated using ImageJ (https://imagej.nih.gov/ij/, accessed on 23 April 2022), and the number of fibers with internal nuclei was determined through the Cell Counter ImageJ plugin. The spleen and a dorsal skin fragment were fixed in 4% paraformaldehyde for 24 h, embedded in paraffin, and 5 µm sections were stained with H&E. The megakaryocyte number was determined on random images covering 12.3 mm² per spleen using the ImageJ Cell Counter plugin, and the thickness and relative proportion of the subcutaneous fat layer was determined on a 5 mm² skin sample area using the NDP Viewer software (Hamamatsu, Hamamatsu, Japan). All muscle, spleen, and skin sections were imaged with the Nanozoomer 2HT slide scanner (Hamamatsu).

Silva-Rojas R., Pérez-Guàrdia L., Lafabrie E., Moulaert D., Laporte J, & Böhm J. (2022). Silencing of the Ca2+ Channel ORAI1 Improves the Multi-Systemic Phenotype of Tubular Aggregate Myopathy (TAM) and Stormorken Syndrome (STRMK) in Mice. International Journal of Molecular Sciences, 23(13), 6968.

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Histological Analysis of Diaphragm and TA Muscles

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Diaphragm was fixed in formaldehyde and embedded in paraffin, and 8 µm transversal and longitudinal sections were stained with hematoxylin and eosin (H&E).
TA muscles were frozen in liquid nitrogen-cooled isopentane and stored at −80 °C. 8 µm transversal sections were stained with H&E, succinate dehydrogenase (SDH), reduced nicotinamide adenine dinucleotide (NADH) and Periodic Acid-Schiff (PAS) to assess muscle fiber morphology, nuclear position, and distribution of mitochondria, reticulum, and glycogen. The images were recorded using the Nanozoomer 2HT slide scanner (Hamamatsu). Myofiber segmentation was performed using Cellpose 1.0 algorithm^{60 (link)} and LabelsToROIS Fiji plugin^{61 (link)} minimum Feret diameter (MinFeret) calculated using ImageJ. Fibers with mislocalized nuclei (centralized or internalized) and fibers with abnormal SDH distribution were counted using Cell Counter ImageJ plugin. Minimum of 800 fibers were measured for each muscle section and a percentage was calculated for each mouse.

Gómez-Oca R., Edelweiss E., Djeddi S., Gerbier M., Massana-Muñoz X., Oulad-Abdelghani M., Crucifix C., Spiegelhalter C., Messaddeq N., Poussin-Courmontagne P., Koebel P., Cowling B.S, & Laporte J. (2022). Differential impact of ubiquitous and muscle dynamin 2 isoforms in muscle physiology and centronuclear myopathy. Nature Communications, 13, 6849.

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