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Human specific pcr primers

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Human-specific PCR primers are short DNA sequences designed to selectively amplify specific regions of the human genome during polymerase chain reaction (PCR) processes. These primers enable the targeted detection and analysis of human genetic material in various applications.

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Lab products found in correlation

Reverse transcription kit, by Bioneer (2 mentions) Real time system, by Thermo Fisher Scientific (2 mentions) Rnaprep mini kit, by Qiagen (2 mentions) Sybr green, by Thermo Fisher Scientific (2 mentions)

2 protocols using human specific pcr primers

1

Quantitative Gene Expression Analysis

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Total RNA was extracted from the patient’s tissue (RNAprep Mini kit, Qiagen), and 500 ng RNA was subjected to reverse transcription using a reverse transcription kit (Bioneer). Real-time quantitative PCR amplification was performed with SYBR Green (ABI) in a real-time system (ABI, USA). Human-specific PCR primers (Bioneer) were used to analyze the expression of the following genes: PDGFRA and GAPDH. The mRNA levels of the specific genes were calculated as ΔΔCt and normalized to those of GAPDH.
Kim T.W., Hong H.K., Lee C., Kim S., Lee W.Y., Yun S.H., Kim H.C., Huh J.W., Park Y.A., Joung J.G., Park W.Y, & Cho Y.B. (2021). The role of PDGFRA as a therapeutic target in young colorectal cancer patients. Journal of Translational Medicine, 19, 446.
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2

Epithelial-Mesenchymal Transition Gene Expression Analysis

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Total RNA was extracted from patient tissue (RNAprep Mini kit, Qiagen), and 500 ng RNA was subjected to reverse transcription using reverse transcription kit (Bioneer). Real-time quantitative PCR amplification was performed with a SYBR Green (ABI) in a real-time system (ABI, USA). Human-specific PCR primers (Bioneer) were used to analyze expression of the following genes: TWIST1, SNAI1, SNAI2, ZEB1, and GAPDH. mRNA levels of specific genes were calculated as ΔΔCt and normalized to GAPDH.
Nam J.Y., Oh B.Y., Hong H.K., Bae J.S., Kim T.W., Ha S.Y., Park D., Lee W.Y., Kim H.C., Yun S.H., Park Y.A., Joung J.G., Park W.Y, & Cho Y.B. (2018). Molecular Characterization of Colorectal Signet-Ring Cell Carcinoma Using Whole-Exome and RNA Sequencing. Translational Oncology, 11(4), 836-844.
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