Bathophenanthrolinedisulfonic acid bps

Manufactured by Merck Group

Sourced in United States

Bathophenanthrolinedisulfonic acid (BPS) is a chemical compound used in laboratory applications. It functions as a metal chelator, capable of forming stable complexes with various metal ions. This property makes BPS a useful tool in analytical and research applications where the detection or quantification of specific metal ions is required.

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Lab products found in correlation

Bathocuproinedisulfonic acid bcs, by Merck Group (1 mentions) Bx51 fluorescence microscope, by Olympus (1 mentions) Fe 2 so4, by Merck Group (1 mentions)

3 protocols using bathophenanthrolinedisulfonic acid bps

Yeast Growth Under Low Copper/Iron

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For growth under low copper conditions, yeast strains were grown in low copper complete minimal media. To attain low copper conditions, the media contained yeast nitrogen base without copper and iron (YNB-CuSO₄-FeCl₃) and 100 μM Bathocuproinedisulfonic acid (BCS; Sigma-Aldrich). To achieve low iron conditions, the media was prepared as described above for low copper replacing 100 μM BCS with 100 μM Bathophenanthrolinedisulfonic acid (BPS; Sigma-Aldrich). Glassware used in these experiments was soaked in 10% nitric acid overnight to remove trace amounts of copper and iron. All yeast cells used for low copper and iron were initially grown to saturation in complete minimal media then sub-cultured into copper or iron deficient media in acid washed glassware.

Wong A., Lam E.M., Pai C., Gunderson A., Carter T.E, & Kebaara B.W. (2021). Variation of the response to metal ions and nonsense-mediated mRNA decay across different Saccharomyces cerevisiae genetic backgrounds. Yeast (Chichester, England), 38(9), 507-520.

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Iron Regulation of Yeast Metabolism

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2.1. Yeast strains, plasmids and growth conditions. The yeast strains used in this study are listed in Table S1. The pCM64-PACO1-lacZ reporter plasmid was made by the insertion of a 613-bp PCR amplified fragment from the ACO1 promoter region into the BglII-BamHI sites of pCM64 vector (from Charles Moehle). The Sfp1-GFP plasmid was constructed by amplifying SFP1 sequence flanked by SalI and SmaI restriction sites, digestion and cloned into pUG35 plasmid. Yeast W303 cells were cultivated in 600 mL of SC medium for at least 15 hours to reach early exponential phase (OD600 = 0.2). Then, 100 μM of the Fe 2+ -specific chelator bathophenanthroline disulfonic acid (BPS; Sigma) was added, and different aliquots were isolated at 0, 10, 30, 90, 180 and 360 minutes after iron limitation. In the case of yeast cells from the BY4741 or other backgrounds, 3 mL overnight precultures were used to inoculate 50 mL of SC (+Fe) or SC + 100 μM BPS cultures (-Fe), and 10 mL aliquots were taken at the specified times. For microscopy, cultures were grown at 30ºC in SC to OD600 = 0.6. Then, yeast cells were washed four times in either SD or SD + 100 μM BPS. Half of the cells were transferred to fresh SD medium or alternatively to SD + 100 μM BPS (-Fe) at OD600 = 0.2. Cultures were grown for 8 hours in continuous shaking. Samples were taken for observation in an Olympus Bx51 fluorescence microscope.

Romero A.M., Ramos-Alonso L., Montellá-Manuel S., García-Martínez J., de la Torre-Ruiz M.Á., Pérez-Ortín J.E., Martínez-Pastor M.T, & Puig S. (2019). A genome-wide transcriptional study reveals that iron deficiency inhibits the yeast TORC1 pathway. Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(9).

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Antifungal Susceptibility Testing Protocol

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Methylene blue was from Cicarelli Labs (Santa Fe, Argentina). Analytical and HPLC grade solvents were from Sintorgan Labs (Buenos Aires, Argentina). Bathophenanthroline disulfonic acid (BPS) and FLU were from Sigma-Aldrich (MO, USA). Fe 2 SO 4 was from Merck (Darmstadt, Germany). Yeast extract-peptone dextrose (YEPD), Sabouraud dextrose (SD) medium, yeast extract, proteose-peptone No. 3 and agar were from Britania Labs (Buenos Aires, Argentina). CAS (Cancidas R , Caspofungin acetate) was from Merck Sharp & Dohme (NJ, USA). RPMI 1640 medium was from Microvet Labs (Buenos Aires, Argentina).
CHROMagar Ò Candida was from CHROMagar Microbiology, France.

Soberón J.R., Lizarraga E.F., Sgariglia M.A., Carrasco Juárez M.B., Sampietro D.A., Ben Altabef A., Catalán C.A, & Vattuone M.A. (2015). Antifungal activity of 4-hydroxy-3-(3-methyl-2-butenyl)acetophenone against Candida albicans: evidence for the antifungal mode of action. Antonie van Leeuwenhoek, 108(5).

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