Lactate dehydrogenase ldh detection kit

Camphorquinone (CQ) (purity, 97%), vinyl acetate (VAc) (purity, 99%), and 2-hydroxyethyl methacrylate (HEMA) (purity, 99%) were supplied by Sigma Aldrich (KGaA, Germany). Mevacor (MVR) was provided from TCI America (Portland, OR, USA). Before usage, monomers were held under nitrogen gas after being purified by distillation at a reduced pressure. Photo-initiator (CQ) was used without purification. The human epithelial cell (HEC) line, GMSM-K prepared by Gilchrist et al. (2000) was offered by Dr Grenier of Laval Universitéy (Quebec, QC, Canada). Dulbecco’s Modified Eagle’s Medium (DMEM) was provided from Corning, Manassas, VA, USA. Fetal bovine serum (FBS) was purchased from Gibco (New York, NY, USA) and 1% of penicillin/streptomycin solution was supplied from Sigma Aldrich (St. Louis, MO, USA). A lactate dehydrogenase (LDH) detection kit was provided by BioVision (BioVision, Milpitas, CA, USA).

Camphorquinone (CQ) (purity, 97%), vinyl acetate (VAc) (purity, 99%), and 2-hydroxyethyl methacrylate (HEMA) (purity, 99%) were supplied by Sigma Aldrich (KGaA, Germany). Mevacor (MVR) was provided from TCI America (Portland, OR, USA). Before usage, monomers were held under nitrogen gas after being purified by distillation at a reduced pressure. Photo-initiator (CQ) was used without purification. The human epithelial cell (HEC) line, GMSM-K prepared by Gilchrist et al. (2000) was offered by Dr Grenier of Laval Universitéy (Quebec, QC, Canada). Dulbecco’s Modified Eagle’s Medium (DMEM) was provided from Corning, Manassas, VA, USA. Fetal bovine serum (FBS) was purchased from Gibco (New York, NY, USA) and 1% of penicillin/streptomycin solution was supplied from Sigma Aldrich (St. Louis, MO, USA). A lactate dehydrogenase (LDH) detection kit was provided by BioVision (BioVision, Milpitas, CA, USA).

2-hydroxyethylmethacrylate (HEMA) (purity, ≥99%), vinyl acetate (Vac) (purity, ≥99%) and camphorquinone (CQ) (purity, 97%) were provided by Sigma Aldrich (Merck, Germany). Acyclovir (Zovirax 800 mg) (ACVR) tablet was purchased from Riyadh Pharma (Riyadh, Saudi Arabia). Monomers were purified by distillation under reduced pressure and stored under nitrogen gas before use. Photoinitiator was used without purification. An 800 mg amount of Zovirax was finely ground using a ceramic mortar and then dissolved in the monomer mixture. The solution obtained was then filtered from the solid residues on Whatman 01 filter paper. The human epithelial cell line (HECL) GMSM-K, constructed by Gilchrist et al. (2000), was provided by Dr. Grenier of Université Laval. Dulbecco’s Modified Eagle’s Medium (DMEM) was purchased from Corning, Manassas, VA, USA. Fetal bovine serum (FBS) was provided by Gibco (New York, NY, USA), and 1% penicillin/streptomycin solution was purchased from Sigma Aldrich (St. Louis, MO, USA). Lactate dehydrogenase (LDH) detection kit was obtained from BioVision (BioVision, Milpitas, CA, USA).