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Fluorescent licor irdye 700dx

Manufactured by LI COR
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The Fluorescent LiCor IRDye 700DX is a near-infrared fluorescent dye. It is designed for fluorescence-based applications, including Western blotting, in-cell Western assays, and other fluorescence detection methods.

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Lab products found in correlation

Fluorescent licor irdye 800cw, by LI COR (2 mentions) Mab 6c5 to gapdh, by Santa Cruz Biotechnology (1 mentions) D1r1r, by Cell Signaling Technology (1 mentions)

2 protocols using fluorescent licor irdye 700dx

1

Alpha-synuclein Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
As primary antibodies we used mAb Syn1 to detect rat endogenous αS (Becton-Dickinson), mAb MJFR1 to detect only human αS (Abcam), mAb EP1537Y to βS (Abcam), mAb 6C5 to GAPDH (Santa Cruz), and pAb C4731 to Calnexin (Sigma). Secondary antibodies were anti-rabbit Fluorescent LiCor IRDye 800CW and anti-mouse Fluorescent LiCor IRDye 700DX.
Ramalingam N, & Dettmer U. (2021). Temperature is a key determinant of alpha- and beta-synuclein membrane interactions in neurons. The Journal of Biological Chemistry, 296, 100271.
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2

Comprehensive Antibody Validation for α-Synuclein

Check if the same lab product or an alternative is used in the 5 most similar protocols
αS (BD Biosciences, Syn1, 610787, Cell Signaling, D37A6); αS pS129 (Abcam, EP1536Y, ab51253; Abcam, MJF-R13, ab168381; Cell Signaling, D1R1R, 23706S – EP1536Y was used throughout the study unless otherwise stated), calnexin (Sigma #C4731), αS pY39 (Biolegend, A15119B), αS pY125 (Abcam, ab131466), αS Y136 (Abcam, ab194775), GAPDH (Santa Cruz, 6C5, sc-32233 and Abcam, 6C5, ab8245), Plk2 (Abclonal #A7066), c-fos (Abcam, 2H2, ab208942). Secondary antibodies were anti-rabbit Fluorescent LiCor IRDye 800CW and anti-mouse Fluorescent LiCor IRDye 700DX. Antibodies used for immunofluorescence and in vitro kinase assays are listed below in the respective sections.
Ramalingam N., Jin S.X., Moors T.E., Fonseca-Ornelas L., Shimanaka K., Lei S., Cam H.P., Watson A.H., Brontesi L., Ding L., Hacibaloglu D.Y., Jiang H., Choi S.J., Kanter E., Liu L., Bartels T., Nuber S., Sulzer D., Mosharov E.V., Chen W.V., Li S., Selkoe D.J, & Dettmer U. (2023). Dynamic physiological α-synuclein S129 phosphorylation is driven by neuronal activity. NPJ Parkinson's Disease, 9, 4.
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