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Cd57 nk 1

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CD57 (NK-1) is a lab equipment product that functions as a marker for natural killer cells. It is used to identify and quantify natural killer cells in biological samples.

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Lab products found in correlation

Facscanto 2 flow cytometer, by BD (2 mentions) Cd19 sj25c1, by BD (2 mentions) Cd8 sk1, by BD (2 mentions) Gallios flow cytometer, by Beckman Coulter (2 mentions) Cd3 sk7, by BD (2 mentions) Cd38 hit2, by BD (2 mentions) Igd ia6 2, by BD (2 mentions) Cd62l dreg 56, by BD (2 mentions) Cd45ra hi100, by BD (2 mentions) Cd31 wm59, by Thermo Fisher Scientific (2 mentions) Cd45ro uchl1, by BD (2 mentions) Cd21 b ly4, by BD (2 mentions) Igm mhm 88, by BioLegend (2 mentions) Cd10 hi10a, by BD (2 mentions) Cd138 b b4, by Bio-Rad (2 mentions) Iga g20 359, by BD (2 mentions) γδ tcr b1, by Thermo Fisher Scientific (2 mentions) αβ tcr ip26, by Thermo Fisher Scientific (2 mentions) Foxp3 pch101, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

CD57 (APC/clone NK-1) (2 citations) CD57 (clone NK-1), (2 citations) Anti-CD57 (NK-1) (2 citations)

3 protocols using cd57 nk 1

1

Comprehensive Immunophenotyping of PBMCs

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Peripheral blood mononuclear cells (PBMCs) were isolated through a Ficoll step gradient and stained for cell surface markers with the following fluorochrome-conjugated antibodies: CD38 (HIT-2, BD), IgD (IA6-2, BD), IgM (MHM-88, BioLegend), CD10 (HI10a, BD), CD19 (SJ25C1, BD), CD21 (B-ly4, BD), CD27 (0323, eBiosience), CD138 (B-B4, AbD SeroTec), IgG (SouthernBioTech #2043-09), IgA (G20-359, BD), CD3 (SK7, BD), CD28 (CD28.2, BD), FOXP3 (PCH101, eBioscience), CD8 (SK1, BD), CD4 (RPA-T4, eBioscience), γδ-TCR (B1.1, eBioscience), αβ-TCR (IP26, eBioscience), CD45RO (UCHL1, BD), CD45RA (HI100, BD), CD57 (NK-1, BD), CD31 (WM59, eBioscience), CD62L (DREG-56, BD), CD152/CTLA4 (BNI3, BD). Samples were acquired on a FACS-Canto II flow cytometer (BD) or on a Gallios flow cytometer (Beckman Coulter, Miami, FL) and analyzed using FlowJo version 7.6.5 analysis software (Treestar, Ashland, OR).
Schubert D., Bode C., Kenefeck R., Hou T.Z., Wing J.B., Kennedy A., Bulashevska A., Petersen B.S., Schäffer A.A., Grüning B.A., Unger S., Frede N., Baumann U., Witte T., Schmidt R.E., Dueckers G., Niehues T., Seneviratne S., Kanariou M., Speckmann C., Ehl S., Rensing-Ehl A., Warnatz K., Rakhmanov M., Thimme R., Hasselblatt P., Emmerich F., Cathomen T., Backofen R., Fisch P., Seidl M., May A., Schmitt-Graeff A., Ikemizu S., Salzer U., Franke A., Sakaguchi S., Walker L.S., Sansom D.M, & Grimbacher B. (2014). Autosomal-dominant immune dysregulation syndrome in humans with CTLA4 mutations. Nature medicine, 20(12), 1410-1416.
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2

Comprehensive Immunophenotyping of PBMCs

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Peripheral blood mononuclear cells (PBMCs) were isolated through a Ficoll step gradient and stained for cell surface markers with the following fluorochrome-conjugated antibodies: CD38 (HIT-2, BD), IgD (IA6-2, BD), IgM (MHM-88, BioLegend), CD10 (HI10a, BD), CD19 (SJ25C1, BD), CD21 (B-ly4, BD), CD27 (0323, eBiosience), CD138 (B-B4, AbD SeroTec), IgG (SouthernBioTech #2043-09), IgA (G20-359, BD), CD3 (SK7, BD), CD28 (CD28.2, BD), FOXP3 (PCH101, eBioscience), CD8 (SK1, BD), CD4 (RPA-T4, eBioscience), γδ-TCR (B1.1, eBioscience), αβ-TCR (IP26, eBioscience), CD45RO (UCHL1, BD), CD45RA (HI100, BD), CD57 (NK-1, BD), CD31 (WM59, eBioscience), CD62L (DREG-56, BD), CD152/CTLA4 (BNI3, BD). Samples were acquired on a FACS-Canto II flow cytometer (BD) or on a Gallios flow cytometer (Beckman Coulter, Miami, FL) and analyzed using FlowJo version 7.6.5 analysis software (Treestar, Ashland, OR).
Schubert D., Bode C., Kenefeck R., Hou T.Z., Wing J.B., Kennedy A., Bulashevska A., Petersen B.S., Schäffer A.A., Grüning B.A., Unger S., Frede N., Baumann U., Witte T., Schmidt R.E., Dueckers G., Niehues T., Seneviratne S., Kanariou M., Speckmann C., Ehl S., Rensing-Ehl A., Warnatz K., Rakhmanov M., Thimme R., Hasselblatt P., Emmerich F., Cathomen T., Backofen R., Fisch P., Seidl M., May A., Schmitt-Graeff A., Ikemizu S., Salzer U., Franke A., Sakaguchi S., Walker L.S., Sansom D.M, & Grimbacher B. (2014). Autosomal-dominant immune dysregulation syndrome in humans with CTLA4 mutations. Nature medicine, 20(12), 1410-1416.
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3

Flow Cytometry Immunophenotyping Protocol

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The following monoclonal antibodies were purchased from BD Biosciences: CD3 (SK7), CD16 (3G8), CD56 (B159), and CD57 (NK-1). The following blocking antibodies were obtained from Biolegend: anti-CD16 (3G8), anti-CD178 (FasL) (NOK-1), anti-CD253 (TRAIL) (RIK-2), anti-CD314 (NKG2D) (MOPC-21). Surface staining was performed by incubation in the dark at 4 o C for 10 min with fluorochromeconjugated mAbs, washed and re-suspended in FACS buffer. DAPI (Life Technologies) was added to each sample at a concentration of 1/200 prior to acquisition. Cells were blocked by incubation for 30 minutes at RT with optimised blocking antibody concentration, alongside isotype control. The LSR Fortessa (Becton Dickinson) cell analyser was used to acquire data. FlowJo software (Tree Star) was used for data analysis.
Domogala A., Blundell M., Thrasher A., Lowdell M.W., Madrigal J.A, & Saudemont A. (2017). Natural killer cells differentiated in vitro from cord blood CD34+ cells are more advantageous for use as an immunotherapy than peripheral blood and cord blood natural killer cells. Cytotherapy, 19(6).
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