Dilp2 gal4

Manufactured by Bloomington Drosophila Stock Center

Sourced in Switzerland

The Dilp2-GAL4 is a genetic tool used in Drosophila research. It is a GAL4 driver line that expresses the GAL4 transcription factor under the control of the Dilp2 (Drosophila insulin-like peptide 2) gene promoter. This allows for the targeted expression of genes of interest in insulin-producing cells in the Drosophila nervous system.

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6 protocols using dilp2 gal4

Genetic Tools for Drosophila Research

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If not stated otherwise, flies were kept at 25°C on either PF or YF under a 12hr light/12hr dark cycle. UAS:lrp1RNAi and UAS:lrp2RNAi lines are from Vienna Drosophila RNAi Center, and oregonRC, repo-Gal4, dilp2-Gal4, dilp2, UAS:gfpRNAi, UAS:GCaMP, UAS:trpA1 lines are from Bloomington Stock center. Published are UAS:ltpRNAi, ltp (Palm et al., 2012 (link)), imp-l2-Gal4 (Bader et al., 2013 (link)), lpp-Gal4 (Brankatschk and Eaton, 2010 (link)), lpr1, lpr2 (Khaliullina et al., 2009 (link)), lrp1, lrp2 (Riedel et al., 2011 (link)), moody-Gal4 (gift from C Klaembt), impl2-Gal4 (gift from E Hafen). UAS:cherry and rab3-Gal4 were generated by transforming VK37 or VK33 flies (Venken and Bellen, 2005 (link)) with our cloned constructs. Yrab3 and Yrab27 are N-terminally YFP^MYC tagged viable rab alleles (rab3 and rab27) generated by targeted YFP^MYC integration into the endogenous locus (publication in preparation).

Brankatschk M., Dunst S., Nemetschke L, & Eaton S. (2014). Delivery of circulating lipoproteins to specific neurons in the Drosophila brain regulates systemic insulin signaling. eLife, 3, e02862.

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Drosophila Genetic Resource Management

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Fly strains were from the following labs/stock center and I appreciate their generosity. dILP2-GAL4, PDF-GAL4, R5710-GAL4, Repo-GAL4 and Repo-GAL80 (Bloomington Stock Center); dILP3-GAL4^{80 (link)}; UAS-dILP5 (Ernst Hafen, Switzerland), UAS-hts-RD (Takashi Suzuki, Japan); UAS-hts-RNAi (GD29102), UAS-hts-RP-RNAi (KK113568), UAS-CalpA-RNAi (GD12275), UAS-dILP5-RNAi (GD16039) and fTRG-585^{48 (link)} (genomic-hts, VDRC 318186) (VDRC); NP3613 (Kyoto Stock Center) and C00257 (Harvard). All stocks were backcrossed to wBerlin for five generations to remove unlinked modifiers and normalize the genetic background.

, & Eddison M. (2021). A genetic screen for Drosophila social isolation mutants and analysis of sex pistol. Scientific Reports, 11, 17395.

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Drosophila Genetic Manipulation Toolkit

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Fly strains were maintained on a standard diet at 25 ℃, 12 light:12 dark cycles and 70% humidity. Actin-GeneSwitch (#9381), Dilp2-Gal4 (#37516), UAS-dSec16 RNAi (#53917), Ok107-Gal4 (#854), 104y-Gal4 (#81014), Dsk-Gal4 (#51981), Dh44-Gal4 (#51987), Lk-Gal4 (#51992), OK371-Gal4 (#26160), Chat-Gal4 (#6793), 55D01-Gal4 (#39110), Ppk-Gal4 (#79278) and Pdf-Gal4 (#6899) were purchased from the Bloomington Stock Center.

Zhang R.X., Li S.S., Li A.Q., Liu Z.Y., Neely G.G, & Wang Q.P. (2022). dSec16 Acting in Insulin-like Peptide Producing Cells Controls Energy Homeostasis in Drosophila. Life, 13(1), 81.

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Drosophila Neuronal Lineage Tracing

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ple-GAL4 (BDSC8848, RRID:BDSC_8848), VGlut^OK371-GAL4 (BDSC26160, RRID:BDSC_26160), ChAT^7.4-GAL4 (BDSC56500, RRID:BDSC_56500), tdc2-GAL4 (BDSC9313, RRID:BDSC_9313), dilp2-GAL4 (BDSC37516, RRID:BDSC_37516), and HMS01544 (BDSC36129, RRID:BDSC_36129) were from Bloomington Drosophila Stock Center. elav-GAL4 was a gift from J. Treisman. PLA2G6 null mutants and isogenic controls were generated in the Steinhauer lab (J.S., in preparation).
Drosophila were cultured on standard media containing 3.83% molasses, 1.58% yeast, and 3.83% corn meal, supplemented with 0.11% methyl paraben and 0.38% propionic acid as mold inhibitors.

Delventhal R, & Steinhauer J. (2020). A course-based undergraduate research experience examining neurodegeneration in Drosophila melanogaster teaches students to think, communicate, and perform like scientists. PLoS ONE, 15(4), e0230912.

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Drosophila Circadian Rhythm Regulation

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The UAS-dbt^RNAi (VDRC: v9241) line was from the Vienna Drosophila RNAi center; the dbt mutants, including dbt^S, dbt^L and the UAS-dbt^K/R line were previously described [8 (link),11 (link),31 (link)]; the UAS-dicer (I); tim-GAL4 line was previously described [32 (link)]; the UAS-dicer (II) (BDSC: 24650), dilp2-GAL4 (BDSC: 37516), 23E10-GAL4 (BDSC: 49032), EB-GAL4(BDSC: 44409), Cre;D/Sb (BDSC: 851) and tub-GAL80ts (BDSC: 7108) were from Bloomington Stock Center; the tim-GAL4 (BDSC: 7126) and per-GAL4 (BDSC: 7127) drivers were previously reported [22 (link),33 (link)]; the repo-GAL4 was provided by Dr. Yi Rao (Peking University), the C309 [24 (link),34 ,35 (link)] and 201Y [24 (link),36 (link)] lines were provided by Dr. Yan Li (Institute of Biophysics, Chinese Academy of Science); UAS-CD8: mGFP (BDSC: 5137) [37 (link)], C205 [38 (link)], 44409 [39 (link)], C232 and C819 [40 (link)] were provided by Dr. Li Liu (Institute of Biophysics, Chinese Academy of Science). The mutants dbt^S and dbt^L have been backcrossed with Canton-S for 6 generations and all experiments have been repeated at least 3 times in the manuscript.

Wang J., Fan J.Y., Zhao Z., Dissel S, & Price J. (2022). DBT affects sleep in both circadian and non-circadian neurons. PLoS Genetics, 18(2), e1010035.

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Drosophila Fly Line Acquisition for Research

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We ordered the following fly lines from the Bloomington Drosophila Stock Center (BDSC): C767-GAL4 (RRID:BDSC_30848), UAS-Dicer2 (RRID:BDSC_24650 and RRID:BDSC_24651), UAS-nlsGFP (RRID:BDSC_7032), UAS-mCD8::GFP (RRID:BDSC_5130), and DILP2-GAL4 (RRID:BDSC_37516). We ordered DH44-GAL4 (VT ID 039046) from the Vienna Drosophila Resource Center (VDRC) [25 ]. SIFa-GAL4 [8 (link)], kurs58-GAL4 (FBti0017957) [26 (link)] and Dilp2mCherry (FBti0202307) [5 (link)] were gifts from Amita Sehgal. C929-GAL4 (FBti0004282) [27 (link)] was a gift from Paul Taghert. We obtained RNAi lines for behavioral screening from the VDRC and the BDSC (see S1 File for a complete list of RNAi lines) [28 (link), 29 (link)].

Ruiz D., Bajwa S.T., Vanani N., Bajwa T.A, & Cavanaugh D.J. (2021). Slowpoke functions in circadian output cells to regulate rest:activity rhythms. PLoS ONE, 16(3), e0249215.

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