The slides were digitized using NanoZoomer 2.0-HT: C9600-13 scanner (Hamamatsu Photonics, Iwata City, Japan) at 20X magnification. For Ki-67, CD31, and CD3 staining, six peripheral/ invasive tumor edge regions were sampled with a fixed field of view of 0.427 mm2 per immunostained slide [16 (link)]. Areas of necrosis were discarded during the evaluation and the senior histopathologist (VP) was blinded to the experimental treatment. ImmunoRatio software was used to quantify Ki67 and CD3 immunoreactivity, and CD31 positively stained endothelial capillaries were manually enumerated. The Ki-67 Labeling Index (LI) was calculated as a percentage of positively stained cells per the total number of tumor cells considered for evaluation.
Nanozoomer 2.0 ht c9600 13 scanner
Manufactured by Hamamatsu Photonics
Sourced in Japan, Germany
The NanoZoomer 2.0-HT: C9600-13 is a high-throughput slide scanner produced by Hamamatsu Photonics. The device is designed to digitize glass slides for various applications, including pathology, cytology, and histology. The scanner features a high-resolution imaging system and an automated slide-loading mechanism to enable efficient and rapid scanning of multiple specimens.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nanozoomer 2.0 ht c9600 13 scanner
1
Quantifying Tumor Proliferation and Angiogenesis
2
Histological Tumor Staging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
According to standard procedures at LabPON, formalin fixed and paraffin embedded tissue sections were cut at 2 μm and stained in an automatic stainer with hematoxylin and eosin (H&E) for routine diagnostic purposes. For the present study, a single slide per patient was selected which contained the most invasive part of the tumor and was used in diagnostics to assess the tumor pT-status. Slides were scanned at ×200 total magnification (tissue level pixel size ~0.455 μm/pixel) using a Hamamatsu NanoZoomer 2.0-HT (C9600-13) scanner (Herrsching, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!