Eclipse e 200 model

One day after the third dose, the animals were bled via the retro-orbital plexus for the individual determination of lactate dehydrogenase (LDH) and C-reactive protein (CRP) levels, using analytical kits as recommended by the supplier (Laborclin and Bioclin, Brazil, respectively). Complete blood cell counts were also taken at this time. Whole blood samples were used to evaluate five hematological parameters: white blood cells (WBC), monocytes (MON), lymphocytes (LYM), neutrophils (NEU) and eosinophils (EOS). WBC counts were carried out using a Neubauer chamber and MON, LYM, NEU and EOS counts were performed using a phase contrast microscope (Eclipse E200 model, Nikon) [27] .

The whole larval body was immediately fixed in 10% neutral buffered formalin supplemented with 2% dimethyl sulfoxide for 24 h, then dehydrated in a series of alcohol baths (beginning with 50% and progressing to 100%), cleared in xylol for 4 h, and finally embedded in paraffin. Cross sections were prepared with a microtome (LEICA RM 2016, Leica Microsystems, USA) at a thickness of about 3 μm, stained with hematoxylin and eosin, and analyzed with a fluorescence microscope (Nikon Eclipse E-200 model, Tokyo, Japan).

The entire larval bodies (n = 5) were promptly fixed in 10% neutral buffered formalin with 2% dimethyl sulfoxide supplemented for 24 h and dehydrated in a series of alcohol baths (starting at 50% and progressing to 100%). After cleaning with xylol for 4 h, the sample was embedded in paraffin. Then, hematoxylin and eosin were used to create cross sections with a microtome (LEICA RM 2016, Leica Microsystems, USA). At least 10 paraffin sections in each group were examined under a fluorescence microscope (Nikon Eclipse E-200 model, Tokyo, Japan).