ARG [as L-arginine HCl], CIT [as L-citrulline], 4-(2-Hydroxyethyl)-1 piperazineethanesulfonic acid (HEPES), CaCl2·4H2O, bovine brain calmodulin, (6R)-5,6,7,8-tetrahydro-l-biopterin (BH4), NADPH, ethylene diamine tetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), and L-NG-Nitroarginine methyl ester (L-NAME) were purchased from Sigma (St. Louis, MO). 15N4-ARG [as its HCl, (U-15N4, 98%)], the internal standard for ARG, 13C6-ARG [as ARG:HCl (U-13C6, 98%)], the internal standard for CIT, D4-CIT [as L-citrulline (4,4,5,5-D4, 96.5 %)], and 15N-nitrite [as NaNO2 (15N, 98%+)] were obtained from Cambridge Isotope Laboratories, Inc. These compounds were used without further purification. Cell culture reagents were purchased from Invitrogen.
15n nitrite
Manufactured by Cambridge Isotopes
Sourced in United States
15N-nitrite is a stable isotope-labeled compound used as a research tool in various scientific applications. It provides a nitrogen-15 labelled version of the nitrite anion (NO2-). The core function of 15N-nitrite is to serve as a tracer or label in studies where the fate or behavior of nitrite is of interest, allowing researchers to track and quantify nitrite-related processes.
Automatically generated - may contain errors
Lab products found in correlation
Bovine brain calmodulin, by Merck Group (1 mentions)
L ng nitroarginine methyl ester l name, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions)
Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions)
15n4 arg, by Cambridge Isotopes (1 mentions)
L citrulline, by Cambridge Isotopes (1 mentions)
Cell culture reagents, by Thermo Fisher Scientific (1 mentions)
4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Merck Group (1 mentions)
2 protocols using 15n nitrite
1
Nitric Oxide Synthesis Assay Protocol
2
Wistar Rat Model for Nitric Oxide Study
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The procedures described in this manuscript were approved by the Ribeirao Preto Medical School, University of São Paulo, and followed the guiding principles published by the National Institutes of Health Guide for Care and Use of Laboratory Animals. Male wistar rats (250–400 g) were requested from the colony at University of São Paulo (Ribeirão Preto Campus, Brazil) and maintained on a 12-h light/dark cycle at a room temperature (22–25 °C) with free access to standard rat chow and water.
All reagents used in the present study were purchased from Sigma Chemical Co. (St Louis, MO, USA), except for diethylenetriamine NONOate (DETANONOate; Cayman Chemical; Ann Arbor, MI, USA), and nitrogen-15-labeled nitrite (15N-nitrite; Cambridge Isotopes Laboratories, Inc.; Andover, MA, USA).
All reagents used in the present study were purchased from Sigma Chemical Co. (St Louis, MO, USA), except for diethylenetriamine NONOate (DETANONOate; Cayman Chemical; Ann Arbor, MI, USA), and nitrogen-15-labeled nitrite (15N-nitrite; Cambridge Isotopes Laboratories, Inc.; Andover, MA, USA).
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