Zetaview analysis software

The size distribution and concentration of OMVs were measured by nanoparticle tracking analysis (NTA, Particle Metrix, Germany) and analyzed using Zetaview Analysis software (Vestad et al., 2017 (link)). Briefly, OMV samples were thawed over ice and diluted in distilled water (1:200–1:4,000). One milliliter of diluted sample was injected for quantification of the OMV size distribution and concentration using Zetaview software with the following specific analysis parameters: maximum particle size: 2,000, minimum particle size 0. All measurements were performed at room temperature (24.5^oC ± 0.1).

The concentration and size distribution of the islet-derived exosomes were measured using nanoparticle tracking analysis (ParticleMetrix, Inning am Ammersee, Germany) and analyzed using Zetaview Analysis software. Briefly, human islet-derived exosomes were isolated using the methods described above and maintained at −80 °C until batched analysis. On the day of analysis, samples were thawed over ice and diluted in distilled water (1:3000). One milliliter of diluted sample was injected for the measurement of exosome size and concentration using Zetaview software.