Syringe driven filter

Manufactured by Merck Group

Sourced in United Kingdom

The Syringe-driven filter is a laboratory equipment designed for the filtration of small sample volumes. It is a standalone device that can be easily attached to a syringe for the purpose of filtering liquids prior to analysis or further processing. The filter can remove particulates, microorganisms, or other unwanted components from the sample, ensuring the integrity of the final product.

Automatically generated - may contain errors

Lab products found in correlation

Opti mem, by Thermo Fisher Scientific (2 mentions) Petrolatum, by Merck Group (1 mentions) Whatman filter paper number 1, by GE Healthcare (1 mentions) C18 reverse phase column, by Merck Group (1 mentions) Agilent 1200 series hplc system, by Agilent Technologies (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Nano series, by Malvern Panalytical (1 mentions) Scientz iid, by Ningbo Scientz Biotechnology (1 mentions) X tremegene 9, by Thermo Fisher Scientific (1 mentions) Pcmv vsv g, by Thermo Fisher Scientific (1 mentions) Pcmv dr8, by Thermo Fisher Scientific (1 mentions) Polybrene, by Merck Group (1 mentions) Puromycin, by Merck Group (1 mentions) X tremegene 9, by Roche (1 mentions) Mbptrap column, by Cytiva (1 mentions) Ultrasonics sonifier sfx550, by Emerson (1 mentions) Sf 900 2 medium, by Thermo Fisher Scientific (1 mentions)

9 protocols using syringe driven filter

Extraction and Formulation of Botanical Ingredients

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Rosa multiflora (stem), Lespedeza bicolor (aerial part), Platycladus orientalis (leaves), Castanea crenata (leaves) and Cornus officinalis (fruit) were purchased from the Gyeongbuk Forest Resource Development Institute, the Republic of Korea. The identity of the plants was confirmed by a taxonomist (Dr. Zi-Eum Im) and voucher specimens were deposited (LVPPM 2001–2005) in our laboratory. The dried and crushed parts of each plant were boiled in 30% ethanol (100 g/Liter). The extracts were filtered with Whatman filter paper Number 1 (GE Healthcare, UK Limited, UK), evaporated to dryness, and freeze-dried. A 5% (w/w) and 10% (w/w) ointments of C2RLP were made using petrolatum (Sigma-Aldrich) as a vehicle. For in vitro experiments on various cell lines, the extract was dissolved in the respective media used to grow the cells and then filtered using a 0.22-μm syringe driven filter (Merck Millipore Ltd., Carrigtwohil, Ireland).

Mechesso A.F., Lee S.J., Park N.H., Kim J.Y., Im Z.E., Suh J.W, & Park S.C. (2019). Preventive effects of a novel herbal mixture on atopic dermatitis-like skin lesions in BALB/C mice. BMC Complementary and Alternative Medicine, 19, 25.

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HPLC Analysis of Mimosine in LL Extract

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HPLC analysis was performed following the procedure as previously described [96 (link)] using an Agilent 1200 Series HPLC System (Agilent LabX, Santa Clara, CA, USA) to detect the active compounds in the extract and validate the presence of mimosine. The mobile phases used consisted of 5% acetic acid (A) and 95% acetonitrile (B) run through a reverse phase C18 column (Sigma-Aldrich, USA) (internal diameter: 4.6 mm; height: 250 mm; particle size: 5 mm) with the following elution program: 0–10 min, 70% A isocratic, 10–20 min gradient to 40% A, and 20–30 min 40% A isocratic. The program was set at a 1.0 mL/min flow rate at 30 °C with an injection volume of 20 µL. The analysis was run for 60 min, and the data acquisition was performed at a 367 nm wavelength. The LL extract and mimosine were filtered through a 0.22 µm syringe-driven filter (Merck Millipore, Germany) to avoid the blockage of the column. LL extract alone (1 mg/mL) was first run to analyze the different compound fractions present in the extract. In order to determine the peak that represents mimosine, the standard compound mimosine was used at concentrations of 0.2, 1, and 5 mg/mL, followed by running LL extract (200 µg/mL) spiked with the different concentrations of mimosine.

Widaad A., Zulkipli I.N, & Petalcorin M.I. (2022). Anthelmintic Effect of Leucaena leucocephala Extract and Its Active Compound, Mimosine, on Vital Behavioral Activities in Caenorhabditis elegans. Molecules, 27(6), 1875.

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Polyphenol Preparation and Dilution Protocol

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RCE, a dark green powder under the market name Red clover extract IFL 40 (UPS), was donated by Linnea SA, Lavertezzo Piano, Switzerland. RGSE, a rust-coloured powder sold under market name MegaNatural™-Gold, was donated by Canandaigua Concentrate and Polyphenolics, Divisions of Constellation Brands, Inc, Madera, California, USA. ARE is a dark purple coloured powder, donated by Artemis, International, Inc (Fort Wayne, Indiana, USA). It was processed with water and ethanol as an extract solvent. CUR is an orange-coloured powder, obtained from turmeric by a solvent extraction method (97% natural); it was supplied by Indus Biotech, Pune, Maharashtra, India.
Prior to use, each polyphenol was initially solubilised in DMSO 100 mg/ml (Sigma-Aldrich, Gillingham, UK) and filtered with a 0.22-μm syringe driven filter (Millipore, Watford, Hertfordshire, UK). This stock solution was then serially diluted to 1 mg/ml, in DMSO. This solution was further diluted in complete medium (CM) to attain a final working concentration of 300 μg/ml, reducing the total percentage of DMSO to less than or equal to 1%. Subsequently, concentrations ranging between 1 ng/ml and 250 μg/ml were prepared by diluting the working solution with clear DMEM, for brain tumour and normal brain cells, or with clear RPMI, for the pancreatic cancer cell line.

Rooprai H.K., Lawrence P., Keshavarz S., Yashod P., Gullan R.W., Selway R.P, & Davies D. (2020). DRAQ7 as an Alternative to MTT Assay for Measuring Viability of Glioma Cells Treated With Polyphenols. Anticancer research, 40(10), 5427-5436.

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Lentiviral Transduction of HEK293T Cells

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HEK293T cells were seeded onto 10-cm culture dishes. The cells were transfected one day later by using Lipofectamine^TM 2000 (invitrogen) with lentiviral transducing vector encoding pLKO or shKDM8 and packaging vectors. After 72 h, virus particles were harvested from the medium and filtered through a 0.45-mm syringe-driven filter (Millipore).

Chen T.J., Wang H.J., Liu J.S., Cheng H.H., Hsu S.C., Wu M.C., Lu C.H., Wu Y.F., Wu J.W., Liu Y.Y., Kung H.J, & Wang W.C. (2019). Mutations in the PKM2 exon-10 region are associated with reduced allostery and increased nuclear translocation. Communications Biology, 2, 105.

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Infecting THP-1 Cells with Toxoplasma and Hammondia

Cited 1 time

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Prior to infection, THP-1 cells were seeded at 1 x 10⁵ cells/well in 24-well plates in cDMEM. To prepare parasites for the infections, HFFs monolayers containing parasite sporozoites were scraped, syringe-lysed, and pelleted (in some cases, freshly excysted oocysts were used). After resuspending the pellet in cDMEM, the parasite mixture was filtered through a 5 μM syringe-driven filter (Millipore). THP-1 cells were infected with either T. gondii or H. hammondi at multiplicity of infection (MOI) of 4, 2 or 1.6. Three biological replicates were made for each parasite infection. THP-1 cells were also mock-infected with parasite filtered through a 0.22 μM syringe-driven filter (Millipore). These protocols are described in detail in [50 (link)].

Wong Z.S., Sokol-Borrelli S.L., Olias P., Dubey J.P, & Boyle J.P. (2020). Head-to-head comparisons of Toxoplasma gondii and its near relative Hammondia hammondi reveal dramatic differences in the host response and effectors with species-specific functions. PLoS Pathogens, 16(6), e1008528.

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Dynamic Light Scattering of IN

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For DLS experiments 500 nM BI/D or 3 µM LEDGF/p75 was added to 200 nM IN in the buffer containing 50 mM HEPES, at pH 7.4, 2 mM DTT, 2 mM MgCl₂, and 1 M NaCl (buffer was filtered twice with 0.22 µm syringe driven filter from Millipore). DLS signals were recorded at RT (25 °C) using a Malvern Nano series zetasizer instrument, and particle size distribution was calculated.

Feng L., Dharmarajan V., Serrao E., Hoyte A., Larue R.C., Slaughter A., Sharma A., Plumb M.R., Kessl J.J., Fuchs J.R., Bushman F.D., Engelman A.N., Griffin P.R, & Kvaratskhelia M. (2016). The Competitive Interplay between Allosteric HIV-1 Integrase Inhibitor BI/D and LEDGF/p75 during the Early Stage of HIV-1 Replication Adversely Affects Inhibitor Potency. ACS chemical biology, 11(5), 1313-1321.

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Synthesis of Multifunctional Polymer Nanoparticles

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PEG-PCL (10 mg), cholesterol-PEG-folate (1 mg), CPPO (2 mg), PFPV or PFBT (1 mg), TPP (0.05 mg) were homogeneously dissolved in 1 mL of tetrahydrofuran (THF). The mixture was then rapidly injected into 10 mL of Milli-Q water under sonication with a sonicator probe (SCIENTZ - II D). Afterward, the THF in the mixture was evaporated through a nitrogen flow, and the remaining solution was filtered through a 0.22 μm syringe-driven filter (Millipore). Ultimately, the obtained POCL dispersion was concentrated by ultrafiltration and used immediately in the following experiments. Other types of nanoparticles, such as POCL/CCPO-, POCL/PFPV-, POCL/TPP-, or POCL/FA-, were prepared according to the same procedure, except for the addition of CPPO, PFPV, TPP, or cholesterol-PEG-folate.

Wu M., Wu L., Li J., Zhang D., Lan S., Zhang X., Lin X., Liu G., Liu X, & Liu J. (2019). Self-Luminescing Theranostic Nanoreactors with Intraparticle Relayed Energy Transfer for Tumor Microenvironment Activated Imaging and Photodynamic Therapy. Theranostics, 9(1), 20-33.

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Lentiviral shRNA Production and Transduction

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For virus production, HEK-293 cells (70–80% confluency) were transfected using X-tremeGENE 9 (Roche Diagnostics Ltd., Burgess Hill, UK) according to manufacturer’s manual with 3 μg pCMV-dR 8.91 (packaging vector, Thermo Fisher) + 0.7 μg pCMV-VSV-G (envelope vector, Thermo Fisher) + 3 μg Lentiviral shRNA vectors (three pLKO1-Puro vectors targeting all Fbln2 variants of the mouse under control of the human U6 promotor (Clones: TRCN0000109479, TRCN0000109478 and TRCN0000109476 (Thermo Fisher), or a scr ctrl shRNA pLKO1-Puro control vector (Thermo Fisher)). These were mixed in 100 µl of Opti-MEM (Thermo Fisher), 6 μl X-tremeGENE 9 was added, and the transfection solution applied to the cells. After overnight incubation, the medium was replaced with fresh DMEM/10%FBS medium, and incubated for another 24 hrs, after which the virus-containing medium was collected and filtered using syringe-driven filters (Millipore Ltd., Livingstone, UK). Filtered virus was added to EpH4 cells in a 6-well plate, incubated for 4 hrs at 37 °C in a 5% CO₂ incubator, topped up with DMEM/10% FBS medium + 8 ug/ml polybrene (Sigma) and finally incubated for 24 hrs.
The medium was replaced with fresh medium containing 3 µg/ml puromycin (Sigma) for selection of transduced cells. Cells were routinely passaged with puromycin-medium to maintain the selection.

Ibrahim A.M., Sabet S., El-Ghor A.A., Kamel N., Anis S.E., Morris J.S, & Stein T. (2018). Fibulin-2 is required for basement membrane integrity of mammary epithelium. Scientific Reports, 8, 14139.

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Recombinant Expression of RNF219-C Fragment

Cited 1 time

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An MBP-tagged C-terminal fragment of RNF219 (residues 434-726) was produced in Spodoptera frugiperda Sf21 insect cells using the MultiBac baculovirus expression system as previously described^{25 (link)}. In brief, the Sf21cells were grown to a density of 2 × 10⁶ cells/ml at 27 °C in Sf900II medium (Thermo Fisher Scientific), infected with the V1 RNF219-C stock of baculovirus, and harvested 48 h after they stopped dividing. Cells were resuspended in lysis buffer (50 mM HEPES, 500 mM NaCl, pH 7.5) and lysed using a Branson Ultrasonics Sonifier SFX550. The lysate was cleared by centrifugation at 40,000 g for 1 h at 4 °C and filtered through 0.45 µm syringe-driven filters (Millipore). The cleared and filtered lysate was diluted to 250 mM NaCl before it was loaded onto a 5 ml MBPTrap column (Cytiva). The bound protein was eluted in one step with binding buffer (50 mM HEPES, 200 mM NaCl, pH 7.5) supplemented with 30 mM maltose.

Poetz F., Corbo J., Levdansky Y., Spiegelhalter A., Lindner D., Magg V., Lebedeva S., Schweiggert J., Schott J., Valkov E, & Stoecklin G. (2021). RNF219 attenuates global mRNA decay through inhibition of CCR4-NOT complex-mediated deadenylation. Nature Communications, 12, 7175.

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