Echinococcus granulosus protoscoleces and cyst walls were isolated aseptically from hydatid cysts removed from the liver of infected sheep slaughtered in an abattoir (Xining, Qinghai, China) and fixed in 4% paraformaldehyde for 20 h. Cyst walls were embedded in paraffin, sliced into 3-μm-thick sections, deparaffinized in xylene, dehydrated in ethanol and then incubated with 0.01 M citrate buffer at 95 °C for 30 min for thermal remediation. After three washes with PBS, the tissues were blocked with 5% bovine serum albumin (BSA) in PBS and treated with mouse anti-rEgHK sera or pre-immune mouse sera at a dilution of 1:200, at 37 °C for 1 h, respectively, following which fluorescein isothiocyanate-conjugated goat anti-mouse IgG (1:200 dilution) (Sigma-Aldrich) was added and incubated at 37 °C for a further 1 h. Fluorescence was detected and images were acquired on an immunofluorescence microscope (model IX71; Olympus Corp., Tokyo, Japan ).
Fluorescein isothiocyanate conjugated goat anti mouse igg
Manufactured by Merck Group
Sourced in Japan
Fluorescein isothiocyanate-conjugated goat anti-mouse IgG is a laboratory reagent used for the detection and quantification of mouse immunoglobulin G (IgG) in various analytical techniques. It consists of goat-derived antibodies specific to mouse IgG that are chemically conjugated to the fluorescent dye fluorescein isothiocyanate (FITC).
Automatically generated - may contain errors
Lab products found in correlation
Tetramethylrhodamineisothiocyanate conjugated goat anti rabbit igg, by Merck Group (1 mentions)
Horseradish peroxidase conjugated goat anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Reagents for 2d dige, by GE Healthcare (1 mentions)
Goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Anti human c4d antibody, by Quidel (1 mentions)
Biotin free polymer detection system, by Leica (1 mentions)
4 protocols using fluorescein isothiocyanate conjugated goat anti mouse igg
1
Immunolocalization of Echinococcus granulosus
2
Antibody Sourcing and Utilization
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Most chemicals were purchased from Sigma-Aldrich (St. Louis, MO), except for reagents for 2D-DIGE, which were purchased from GE Healthcare (Uppsala, Sweden). Primary antibodies were purchased from Abcam (Cambridge, UK), except for anti-ARV, which was a kind gift from Dr. L. H. Lee at National Chung Hsing University, Taiwan. Secondary antibodies for immunofluorescence analysis, tetramethylrhodamine isothiocyanate−conjugated goat anti-rabbit IgG and fluorescein isothiocyanate−conjugated goat anti-mouse IgG, were purchased from Sigma-Aldrich. Secondary antibodies for western blotting, horseradish peroxidase−conjugated goat anti-mouse IgG and goat anti-rabbit IgG, were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA).
3
Ultrasound-Guided Allograft Biopsy Protocol
4
Ultrastructural Analysis of PEDV in Vero Cells
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Vero cells infected with the CH/QX-2 isolate were trypsinized at 24 h post infection and centrifuged for 5 min at 800×g. The cell pellets were resuspended in 0.01M PBS (pH 7.2-7.4) and centrifuged for 5 min at 800×g. The resulting pellets were fixed in 2.5% glutaraldehyde in 0.1 M Na cacodylate buffer (pH 7.4) and post-fixed in 1% osmium tetroxide for 90 min. The samples were dehydrated in an ascending ethanol series, followed by propylene oxide and embedded in Eponate 12 TM . Ultrathin sections were stained with uranyl acetate and lead citrate, and examined by TEM (H-7500).
IFA was used to detect PEDV in the fixed-methanol infected Vero cells utilizing a 1:1000 dilution of mouse anti-S monoclonal antibody specific for PEDV (Cat No: 9191, MEDIAN Diagnostics Inc, Korea) and a 1:100 dilution of fluorescein isothiocyanate-conjugated goat anti-mouse IgG (Cat no: F0257, Sigma). Vero cells without virus were similarly treated and used as an antigen negative control. The results were obtained using a fluorescence microscope.
IFA was used to detect PEDV in the fixed-methanol infected Vero cells utilizing a 1:1000 dilution of mouse anti-S monoclonal antibody specific for PEDV (Cat No: 9191, MEDIAN Diagnostics Inc, Korea) and a 1:100 dilution of fluorescein isothiocyanate-conjugated goat anti-mouse IgG (Cat no: F0257, Sigma). Vero cells without virus were similarly treated and used as an antigen negative control. The results were obtained using a fluorescence microscope.
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