Mg149

SR3029, D4476, MG149, NU9056, MG132 and cycloheximide (CHX) were purchased from MedChemExpress (MCE, Monmouth Junction, NJ, USA). The following primary antibodies were used: anti-CK1δ, anti-β-catenin, anti-p300, anti-acetyl-lysine, IgG (Santa Cruz Biotechnology, Heidelberg, Germany), anti-CBP, anti-acetyl-β-catenin (K49), anti-phospho-β-catenin (S45), anti-Flag, anti-V5, anti-CK1ϵ, anti-GFP, anti-mouse IgG (Cell Signaling Technology, Danvers, MA), anti-Tip60 (Abcam, Cambridge, MA, USA), and anti-GAPDH antibody (Proteintech, Chicago, IL, USA). The Tip60-Flag, PCAF-Flag, p300-Flag and pEGFP-N1 plasmids were purchased from Vigenebio (Weizhen, Shandong, China). The SuperTopFlash reporter plasmid was provided by Karl Willert (University of California at San Diego, La Jolla, CA, USA). The expression plasmids encoding β-catenin, pCMXβgal (β galactosidase, β-gal), CK1α-V5, CK1δ-V5, CK1ϵ-V5, CK1α-Flag, CK1δ-Flag, CK1ϵ-Flag, CK1γ-Flag have been described previously (35 (link), 36 (link)). For the construction of GFP-tagged CK1δ, the cDNAs encoding human CK1δ was amplified by PCR and subcloned into the BamHI/EcoRI site of pEGFP-N1 vector using ClonExpress Ultra One Step Cloning Kit (Vazyme, Nanjing, China). The primer sequences used are as follows: CK1δ-GFP-sense, 5’-CTCGAGCTCAAGCTTCATGGAGCTGAGAGTCGGGAAC-3’; CK1δ-GFP-antisense, 5’-CTCACCATAAGGTGGCGACCGGTGTAGGTGCGTCGTG.

Anti-MOF (sc-81765) and breast-cancer susceptibility gene 1 (BRCA1) (Santa Cruz, sc-6954) were obtained from Santa Cruz Biotechnology. Antibodies including CUL4A (14851-1), CUL4B (12916-1), glyceraldehyde-3-phosphate dehydrogenase(GAPDH) (60004-1-Ig), and Flag (66008-2) were purchased from Proteintech (Wuhan, China). Other antibodies were listed as follows: H4K16ac (Epitomics, EPR1004), ERα (Cell Signaling Technology, Inc (CST), #8644), Ki67 (Abcam, ab16667), murine double minute 2 (MDM2) (Wanleibio, WL01906), HSP90 (Sangon Biotech, D120009), HSP90 K294ac (Rockland, 600-401-981), and acetylated lysine (CST, #9441). Inhibitors including MG149, cycloheximide (CHX), and MG132 were purchased from MedChemExpress (MCE, Princeton, NJ, USA). TAM was purchased from Sigma. CHX, MG149, MG132, and TAM were dissolved in dimethyl sulfoxide (DMSO).