Muscular biopsy was performed in the proband of family 2 to observe the ultrastructure of mitochondria in muscular tissue. Muscular tissue was fixed in 2.5% glutaraldehyde (Sangon Biotech, China) in PBS at 4°C for 2 h. Then, the tissue block was washed three times with PBS, and the block embedded with agar were fixed again with 1% osmium tetroxide for 2 h then washed three times with PBS. The tissue block was dehydrated with an ethanol series and fixed again with Epon 812 (Sangon Biotech, China). The ultrathin (70 nm) sections were collected on Formvar/carbon‐coated nickel grids (Sangon Biotech, China), and the grids were stained with 2.5% uranyl acetate (Sangon Biotech, China) for 7 min followed by lead citrate (Sangon Biotech, China) for 2.5 min, and then the sections were observed with a JEM‐1011 electron microscope (JEOL, Japan).
Formvar carbon coated nickel grids
Manufactured by Sangon
Sourced in China
Formvar/carbon‐coated nickel grids are a type of lab equipment used for various microscopy and sample preparation applications. These grids provide a stable and uniform support structure for thin samples, allowing for effective imaging and analysis. The nickel composition and the Formvar/carbon coating work together to enhance the durability and conductivity of the grids, making them suitable for use in electron microscopy and other related techniques.
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2 protocols using formvar carbon coated nickel grids
1
Ultrastructural Analysis of Mitochondria in Muscle Tissue
2
Ultrastructural Analysis of Mitochondria in Muscle Tissue
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Muscular biopsy was performed in the proband of family 2 to observe ultrastructure of mitochondria in muscular tissue. Muscular tissue was fixed in 2.5% glutaraldehyde (Sangon Biotech, China) in PBS at 4°C for 2 h. Then, the tissue block was washed three times with PBS, and the block embedded with agar were fixed again with 1% osmium tetroxide for 2 h then washed three times with PBS. The tissue block was dehydrated with an ethanol series and fixed again with Epon 812 (Sangon Biotech, China). The ultrathin (70 nm) sections were collected on Formvar/carbon-coated nickel grids (Sangon Biotech, China), and the grids were stained with 2.5% uranyl acetate (Sangon Biotech, China) for 7 min followed by lead citrate (Sangon Biotech, China) for 2.5 min, and then the sections were observed with a JEM-1011 electron microscope (JEOL, Japan).
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