Type A gelatin from pigskin was purchased from China National Pharmaceutical Group Corporation (Beijing, China) and used after dialysis. The isoelectric point (pI, 8.5) of the dialyzed gelatin after complete deionization was determined by fluorescence spectroscopy. Sodium dodecyl sulfate (SDS) was purchased from Alfa Aesar and recrystallized from ethanol before use. Ally glycidyl ether (AGE) and chloroplatinic acid hexahydrate (H2Pt6Cl6•6H2O) were obtained from Alfa Aesar. Hexamethylcyclotrisiloxane (D3, >95%), n–butyllithium (C4H9Li, >99%), and chlorodimethylsilane (C2H7ClSi, >99%) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Benzene, deionized water (conductivity = 2.06 µS cm−1, dielectric constant ε = 80.40), methanol (ε = 32.70), ethanol (ε = 24.50), isopropanol alcohol (ε = 17.90), acetone (ε = 20.70), tetrahydrofuran (THF, ε = 7.58), and 1,4-dioxane (ε = 2.25) solvents (China National Pharmaceutical Group Corporation) were all of analytical reagent (AR) grade and strictly dehydrated before use.
Hexamethylcyclotrisiloxane d3
Manufactured by Merck Group
Sourced in United States, Poland
Hexamethylcyclotrisiloxane (D3) is a cyclic siloxane compound. It is a colorless, volatile liquid that is commonly used as a raw material in the production of silicone polymers and other silicone-based products.
Automatically generated - may contain errors
Lab products found in correlation
Sodium dodecyl sulfate (sds), by Thermo Fisher Scientific (1 mentions)
Ethanol, by Sinopharm (1 mentions)
N butyllithium c4h9li, by Merck Group (1 mentions)
Chlorodimethylsilane c2h7clsi, by Merck Group (1 mentions)
Acetone, by Sinopharm (1 mentions)
Methanol, by Sinopharm (1 mentions)
Chloroplatinic acid hexahydrate, by Thermo Fisher Scientific (1 mentions)
Sircol collagen assay, by Biocolor (1 mentions)
2 protocols using hexamethylcyclotrisiloxane d3
1
Gelatin Extraction and Characterization
2
Collagen Isolation and Characterization
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In all experiments, commercially available collagen type I from rat tails was used (Sigma-Aldrich, Poznan, Poland). Hexamethylcyclotrisiloxane (D3) and PDMSs (20, 100, 1000, and 1200 cS were purchased from Sigma–Aldrich, Poznan, Poland). For measuring the native collagen concentration, “Sircol Collagen Assay” (Biocolor, County Antrim, UK) was used. For total collagen concentration measurement by the Lowry method, the phenol Folin-Ciocalteu reagent (Sigma-Aldrich, Poznan, Poland) was used. Collagen purification after fluorescence labeling was performed with the use of Sephadex G-25 Medium bed (GE Healthcare, Warsaw, Poland). Other reagents (unless stated otherwise) were purchased from Sigma–Aldrich (Poznan, Poland).
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