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Rabbit anti zo 1 polyclonal antibody

Manufactured by Cell Signaling Technology
Sourced in United States

Rabbit anti‐ZO‐1 polyclonal antibody is a laboratory reagent used to detect the presence and distribution of the ZO‐1 protein in biological samples. ZO‐1 is a tight junction-associated protein that plays a role in the regulation of cell-cell adhesion and the formation of tight junctions.

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2 protocols using rabbit anti zo 1 polyclonal antibody

1

Antibody Characterization for Cell Signaling

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The human IL‐17A recombinant protein (rhIL‐17A, #8928), rabbit anti‐ZO‐1 polyclonal antibody (#8193), rabbit anti‐MMP‐9 polyclonal antibody (#15561), MMP‐2 antibody (#4022) the rabbit anti‐Slug polyclonal antibody (#9585), the rabbit anti‐AKT polyclonal antibody (#9272), the rabbit anti‐phospho‐AKT(Ser473) polyclonal antibody (#9271), the rabbit anti‐phospho‐PI3K polyclonal antibody (#4249), the rabbit anti‐phospho‐PI3K p85 (Tyr458)/p55 (Tyr199) polyclonal antibody (#4228), the anti‐rabbit IgG HRP‐linked antibody (#7074) and anti‐mouse IgG HRP‐linked antibody (#7076) were purchased from Cell Signaling Technology (Danvers, MA, USA). The mouse anti‐α‐SMA monoclonal antibody (ab7817), the mouse anti‐Twist monoclonal antibody (ab175430), the mouse anti‐Bmi1 monoclonal antibody (ab14389) were obtained from Abcam (Cambridge, UK). The mouse anti‐GAPDH monoclonal antibody was purchased from Beyotime Biotechnology (Shanghai, China). The Alexa Fluor® 488 fluorescent second antibody and CellTracker CM‐Dil (C7000) were purchased from ThermoFisher Scientific (Waltham, MA, USA).
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2

Immunoblotting and Immunofluorescence Assay

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The rabbit anti–CD155 polyclonal antibody (ab103630) and the mouse anti–α‐SMA monoclonal antibody (ab7817) were obtained from Abcam. The rabbit anti–ZO‐1 polyclonal antibody (#8193), the rabbit anti–MMP‐9 polyclonal antibody (#15561), the rabbit anti–Slug polyclonal antibody (#9585), the rabbit anti‐E‐cadherin polyclonal antibody (#3195), the rabbit anti–vimentin polyclonal antibody (#5741), the rabbit anti–ZEB1 polyclonal antibody (#3396), the rabbit anti–TGF‐β polyclonal antibody (#3711), the rabbit anti‐Smad3 polyclonal antibody (#9523), the rabbit anti‐IL‐6 polyclonal antibody (#12153), the rabbit anti‐Stat3 polyclonal antibody (#30835), the rabbit anti–phospho‐Stat3(Tyr705) polyclonal antibody (#9145), the rabbit anti‐Caspase3 polyclonal antibody (#9662), the rabbit anti‐Cleaved‐Caspase3 polyclonal antibody (#9664), the anti–rabbit IgG HRP‐linked antibody (#7074) and anti–mouse IgG HRP‐linked antibody (#7076), were purchased from Cell Signaling Technology. The mouse anti–GAPDH monoclonal antibody was purchased from Beyotime Biotechnology. The Alexa Fluor 488 and 594 fluorescent second antibodies were purchased from ThermoFisher Scientific.
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