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Mouse ifnβ duoset elisa kit

Manufactured by R&D Systems
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Sourced in United States

The Mouse IFNβ DuoSet ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of mouse interferon beta (IFNβ) levels in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Mouse il 6 duoset elisa kit, by R&D Systems (1 mentions) Mouse il 18 duoset elisa kit, by R&D Systems (1 mentions) Microplate reader, by Molecular Devices (1 mentions)

Close spelling variants of this product

Mouse DuoSet ELISA kits DuoSet ELISA kits DuoSet ELISA DuoSet Mouse Mouse ELISA kits DuoSets ELISAs

2 protocols using mouse ifnβ duoset elisa kit

1

Cytokine Quantification by ELISA

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ELISA was used to measure secreted IL-1β, IL-18, IL-6 and IFNβ in cell-free supernatants harvested at indicated time points using R & D Systems Mouse/Human IL-1 beta/IL-1F2 DuoSet ELISA kit, Mouse IL-18 DuoSet ELISA kit, Mouse IL-6 DuoSet ELISA kit and Mouse IFNβ DuoSet ELISA kit respectively. The assay was performed according to manufacturer’s instruction. For determining the cytokine concentration of the samples in 96-well plate absorbance was measured at 450 nm and calculated by GraphPad Prism linear regression analysis.
Rastogi S., Ellinwood S., Augenstreich J., Mayer-Barber K.D, & Briken V. (2021). Mycobacterium tuberculosis inhibits the NLRP3 inflammasome activation via its phosphokinase PknF. PLoS Pathogens, 17(7), e1009712.
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2

Quantifying IFN-β Levels by ELISA

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Culture medium levels of IFN-β were measured by the use of commercially available enzyme-linked immunosorbent assay (ELISA) kit (Mouse IFNβ DuoSet ELISA kit, DY8234-05; R&D Systems, Minneapolis, MN, United States) according to the manufacturer’s instructions. The plate was read on a microplate reader (Molecular Devices, Menlo Park, CA, United States). Assays were performed in duplicate.
Palikhe S., Ohashi W., Sakamoto T., Hattori K., Kawakami M., Andoh T., Yamazaki H, & Hattori Y. (2019). Regulatory Role of GRK2 in the TLR Signaling-Mediated iNOS Induction Pathway in Microglial Cells. Frontiers in Pharmacology, 10, 59.
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