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Anti pe conjugated ferromagnetic beads

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Anti-PE-conjugated ferromagnetic beads are magnetic particles that are coated with anti-phycoerythrin (anti-PE) antibodies. These beads can be used for the detection and isolation of PE-labeled cells or molecules in various applications.

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Lab products found in correlation

Collagenase type 4, by Merck Group (2 mentions) Monocyte colony stimulating factor, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Anti-PE beads (43 citations) Anti-PE ferromagnetic beads (2 citations) Ferromagnetic beads (2 citations)

2 protocols using anti pe conjugated ferromagnetic beads

1

Isolation and Culture of Murine Intestinal Macrophages

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Murine intestinal macrophages were isolated by immunomagnetic cells separation. Briefly, intestinal tissue was digested with HBSS containing 1 mM collagenase type IV (Sigma) × 2 h at 37°C. Macrophages were first stained with phycoerythrin (PE)-conjugated anti-Ly6C and anti-F4/80 antibodies and then separated using anti-PE-conjugated ferromagnetic beads (Miltenyi Biotec, San Diego, CA). More than 90% of these cells were confirmed as CD11b+ F4/80+ CD11cint macrophages by FACS.
BMDMs were prepared (37 (link)) by culturing bone marrow cells in DMEM with 10% FCS and 40 ng/mL monocyte-colony stimulating factor (PeproTech, Rocky Hill, NJ). After 5 days, BMDMs were collected by scraping and cultured in RPMI with 10% FCS.
MohanKumar K., Namachivayam K., Chapalamadugu K., Garzon S.A., Premkumar M.H., Tipparaju S, & Maheshwari A. (2016). Smad7 Interrupts TGF-β Signaling in Intestinal Macrophages and Promotes Inflammatory Activation of these Cells during Necrotizing Enterocolitis. Pediatric research, 79(6), 951-961.
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2

Isolation and Culture of Murine Intestinal Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine intestinal macrophages were isolated by immunomagnetic cells separation. Briefly, intestinal tissue was digested with HBSS containing 1 mM collagenase type IV (Sigma) × 2 h at 37°C. Macrophages were first stained with phycoerythrin (PE)-conjugated anti-Ly6C and anti-F4/80 antibodies and then separated using anti-PE-conjugated ferromagnetic beads (Miltenyi Biotec, San Diego, CA). More than 90% of these cells were confirmed as CD11b+ F4/80+ CD11cint macrophages by FACS.
BMDMs were prepared (37 (link)) by culturing bone marrow cells in DMEM with 10% FCS and 40 ng/mL monocyte-colony stimulating factor (PeproTech, Rocky Hill, NJ). After 5 days, BMDMs were collected by scraping and cultured in RPMI with 10% FCS.
MohanKumar K., Namachivayam K., Chapalamadugu K., Garzon S.A., Premkumar M.H., Tipparaju S, & Maheshwari A. (2016). Smad7 Interrupts TGF-β Signaling in Intestinal Macrophages and Promotes Inflammatory Activation of these Cells during Necrotizing Enterocolitis. Pediatric research, 79(6), 951-961.
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