The human colon adenocarcinoma cell line Caco-2 was obtained from the Cell Biological Institute of Shanghai, Chinese Academy of Sciences (Shanghai, China). The cells were grown at 37°C in air and 5% CO2 in sterile basal medium/DMEM-H (Sigma-Aldrich, Merck KGaA, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (Genview, Beijing, China), 1% L-glutamine, 100 U/ml penicillin, 100 mg/ml streptomycin and 0.25 mg/ml amphotericin B. The culture medium was changed every 2–3 days. Prior to treatment, the cells were plated with fresh medium (1×106 cells/ml) and cultured. On the second day after plating, Caco-2 cells were incubated with different concentrations of H2O2 (100, 200 and 400 μM) and 85% oxygen [cells were cultured in three gas incubators (CB160; Binder GmbH, Tuttlingen, Germany), with 85% oxygen and 5% CO2] for 24 h. A group of control cells received no treatment. Subsequently, the cultured cells were harvested, and the RNA and protein were extracted. All experiments were repeated 6–8 times.
Fetal bovine serum (fbs)
Manufactured by Genview
Sourced in China
Fetal bovine serum is a cell culture supplement derived from the blood of bovine fetuses. It provides a rich source of growth factors, nutrients, and other components essential for the in vitro cultivation of cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using fetal bovine serum (fbs)
1
Caco-2 Oxidative Stress Modeling
2
Isolation of Osteoporotic Osteoblasts
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Osteoporotic osteoblasts were isolated from hip joint of postmenopausal osteoporotic women who were 50 to 55 years old [13 (link)]. Normal osteoblasts were obtained from the healthy postmenopausal women who were 50 to 55 years old. Cells were cultured in α-MEM medium (Gbico) supplemented with 10% fetal bovine serum (GENVIEW), 100 IU/mL penicillin, and 100 μg/mL streptomycin at 37°C, 5% CO2, and a humidified atmosphere.
3
Oxygen-induced Colon Cell Stress
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The human normal colon mucosal epithelial cell line NCM460 (C335) was obtained from the Shanghai Institute of Cell Biological,Chinese Academy of Sciences. The cells were incubated at 37°C in air and 5% CO2 in sterile basal medium/DMEM-H (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (Genview, Beijing, China), 1% L-glutamine, 100 U/ml penicillin, 100 mg/ml streptomycin, and 0.25 mg/ml amphotericin B. The culture medium was changed every 2 to 3 d. Prior to treatment, the cells (1 × 106 cells/ml) were plated with fresh medium and cultured in 37°C in air and 5% CO2 for 24 h. On the second day after plating, the cells were treated with N-acetyl-L-cysteine (NAC) or apocynin (APO) and/or 85% oxygen (three-gas incubator, CB160, BINDER, Neckarsulm, Germany) for 24 h. The control cells received no treatment. Next, we harvested the cultured cells and extracted RNA and protein from the cells. All experiments were repeated 6 to 8 times.
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