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Thinprep cytologic test

Manufactured by Hologic
Sourced in United States

The ThinPrep cytologic test is a laboratory equipment product designed to collect and prepare cervical cell samples for microscopic examination. It is a reliable and accurate tool for screening and detecting abnormal cervical cells that may indicate the presence of precancerous or cancerous conditions.

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4 protocols using thinprep cytologic test

1

Cervical Epithelial Cell Collection

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The clinician used a speculum to expose the cervix and wipe off excessive secretions with a cotton swab. A cervical brush was rotated slowly in one direction for 6–7 times to obtain sufficient cervical epithelial cells; this was repeated twice. One of the cytology brushes was used for HPV-DNA detection, and the other brush was placed in preservation solution for a ThinPrep cytologic test (TCT, Hologic, USA, MA). All samples were stored at 4 ℃ and tested within 48 h.
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2

Cervical Specimen Collection and Cytology Evaluation

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The present study was approved by the Ethics Committee of the School of Medicine, Jinan University (Guangzhou, China). Cervical specimens were collected with a Cytobrush (Qiagen, Inc., Valencia, CA, USA) and placed into a ThinPrep Pap test vial (Hologic, Inc., Marlborough, MA, USA) containing 20 ml PreservCyt Solution (Hologic, Inc.). Referral Pap specimens were used for the ThinPrep cytologic test, which was performed by Kingmed Center for Clinical Laboratory Corporation (Guangzhou, China) using the ThinPrep 2000 System (Hologic, Inc.) and evaluated for routine screening cytology. A Pap smear was positive for a squamous intraepithelial lesion (SIL) if there was a low (LSIL) or high (HSIL) grade SIL, as classified according to the Bethesda Classification System (16 (link)). Excluding cases with other types of uterine disease and without a history of hysterectomy, a total of 164 cases [120 LSIL (73.2%) and 44 HSIL (26.8%)] were recruited. A total of 296 control samples without an intraepithelial lesion or malignancy were recruited from the area of residence of the cases. The study was explained to each individual and written consent for their participation was obtained.
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3

Cervical Cytology Reporting Protocol

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A TCT (Thinprep cytologic test purchased from Hologic.lnc 20160621) test was used to obtain materials for production. The accompanying report was prepared based on The Bethesda System (TBS) for Reporting Cervical Cytology (2014). Abnormal results included negative for intraepithelial lesion or malignant tumor (NILM), atypical squamous cells of undetermined significance (ASCUS), atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion (ASCH), low-grade squamous intraepithelial lesion (LSIL), high grade squamous intraepithelial lesion (HSIL), squamous cell carcinoma (SCC), and atypical glandular cells (AGC). A positive TCT diagnosis included patients with ASCUS, ASC-H, LSIL, HSIL, SCC, or AGC.
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4

Cervical Cytology Testing with ThinPrep

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ThinPrep® Cytologic Test (TCT, Hologic, Marlborough, MA, USA) was used to determine the cervical cytology testing. The Bethesda System (TBS) for reporting cervical cytology in 2014 was applied for TCT report format [17 (link)].
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