Anti rabbit p creb

For immunofluorescence staining, 1×10⁵ cells were fixed with 4% paraformaldehyde for 30 min at room temperature, rinsed in PBS, blocked in 5% BSA-containing TBS-Tx (supplemented with 0.2% Triton-X-100) for 1.5 h at room temperature and incubated with the primary antibodies overnight at 4°C. The primary antibodies used were anti-mouse phospho (p)-p38 (1:200; cat. no. 9216; Cell Signaling Technology, Inc.) and anti-rabbit p-CREB (1:200; cat. no. 9198; Cell Signaling Technology, Inc.). Then, cells were incubated with the secondary antibodies, Alexa Fluor 488 goat anti-mouse IgG (cat. no. A11001; Invitrogen; Thermo Fisher Scientific, Inc.) and Alexa Fluor 488 goat anti-rabbit IgG (cat. no. A11008; Invitrogen; Thermo Fisher Scientific, Inc.) with 4,6-diamidino-2-phenylindole (Sigma-Aldrich; Merck KGaA) for 1 h at room temperature. Immunofluorescence staining was imaged using a ZEISS LSM700 confocal microscope (magnification, ×40).