Total RNAs including MiR-224, miR-215 and miR-143 were extracted from 200 μl serum and from 100 mg tissue, modified procedure of Abbott mSample preparation system kit (Abbott Molecular, Inc., Des Plaines, IL). The spike in C. elegans miR-39 as a synthetic RNA oligo nucleotide (Qiagen, Hilden, Germany) was added to all samples before extraction.
Msample preparation system kit
Manufactured by Abbott
Sourced in United States
The Abbott mSample preparation system kit is a laboratory equipment product designed for sample preparation. It automates the process of extracting and purifying nucleic acids from a variety of sample types. The core function of this system is to facilitate efficient and consistent sample preparation for downstream analytical applications.
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Lab products found in correlation
4 protocols using msample preparation system kit
1
Serum and Tissue Total RNA Extraction
2
RNA Extraction from Serum and Tissue
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Total RNAs were extracted from 200 μl serum according to the manufacturer’s instructions of Abbott mSample preparation system kit (Abbott Molecular, Inc., Des Plaines, IL) and from 100 mg tumor and non-tumor liver tissues according to the manufacturer’s instructions of Promega kit (Promega Corporation, Madison, WI 53711 USA) and the extracted RNA was aliquoted and stored at -80°C until used.
3
SARS-CoV-2 RNA Extraction and Detection
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The automatic extraction and purification of the SARS-COV-2 RNA were performed on Abbott m2000sp System using the Abbott mSample Preparation System kit (Abbott GmbH & Co. KG, Max-Planck-Ring 2, 65,205 Wiesbaden, Germany). The extracted viral RNA was subsequently subjected to real-time reverse-transcription polymerase chain reaction for cDNA synthesis and amplification of target regions using Abbott Real-Time SARS-CoV-2 amplification reagent kit (Abbott Molecular Inc 1300; East Touhy Avenue Des Plaines, IL 60018 USA).
4
HCV Genotyping by Abbott Assays
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For analysis GTs with the Abbott GT II and PLUS assays, RNA was extracted from the 300 μL serum on the Abbott m2000sp system using the Abbott mSample Preparation System kit (Des Plaines, IL, USA) according to the manufacturer’s instruction. Reverse transcription (RT)-PCR master mixes were set up using the Abbott m2000sp and Abbott RealTime HCV Genotype II Amplification Reagent Kit or the RealTime HCV Genotype PLUS Amplification Reagent Kit (Des Plaines, IL, USA). RT-PCR reactions were performed using the Abbott m2000rt instrument.
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