IHC labeling was performed on the Benchmark XT autostainer (Ventana Medical Systems Inc., Tucson, AZ) using the I-View detection kit. The standard antibodies used, vendors, pretreatments, and dilutions were as follows: cathepsin K (Abcam; steam, 1:800), HMB45 (Novacastra; catalog#ncl-hmb45, steam, 1:100), Melan A (Cell Marque; catalog 281 M-8, clone A103, steam, 1:500), Cam5.2 (Cell Marque; steam, prediluted), AE1/3 (Chemicon; steam, 1:4000), MITF (Dako; steam, 1:50), PAX2 (Zymed; catalog#71 to 6000, steam, 1:100), RCC marker antigen (Leica; steam, 1:50), vimentin (Ventana, 790-2917; prediluted), CD10 (Leica; org-8941, steam, prediluted), racemase (Zeta; P504S, steam, 1:100), SOX10 (Santa; SC-17,342, steam, 1:100), EMA (Ventana; 760-4259, stream, prediluted), inhibin (Serotec; steam, 1:25), PAX8 (ProteinTech Group, Chicago, IL; steam, 1:100), CA IX (Novacastra NCL-L-CA IX; steam, 1:100), EpCAM (Santa Cruz; sc-25,308, steam, 1:200), Ksp-cadherin (Invitrogen, San Francisco, CA; steam, 1:100), CD117 (Cell Marque CMA768; steam, prediluted), and ER (Novacastra; 6F11, 1 μg/mL). For pS6, after a 50-minute steam pretreatment in EDTA buffer, we used the antibody from Cell Signaling (#2215) at 1:200 dilution overnight at 4°C, followed by the Dako Polyclonal Envision + secondary for 30 minutes.
Melan a
Manufactured by Cell Marque
Melan A is a lab equipment product used for immunohistochemical detection of melanocytic cells. It serves as a marker for melanoma and other melanocytic lesions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using melan a
1
Comprehensive Immunohistochemical Profiling of Tumors
2
Immunohistochemical Evaluation of Melanoma
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Formalin-fixed and paraffin-embedded sections were serial sectioned at 3 μm.
Commercially available antibodies were used for the immunohistochemistry, with a control section for every batch of 20 sections, and evaluated blinded by a senior pathologist (TS). Ultraview fast red was used as detection system, to minimise the influence of the pigmentation of melanophages or melanoma cells. The sections were stained for cd163+ macrophages (Ab Serotec, EDHu-1, 1:100, cytoplasm) and cd66b+ granulocytes (BD Bioscience, G10F5, 1:200, cytoplasm). For tumour cell proliferation, we used double stains of MelanA (Cell Marque, M2-7C10, 1:50, cytoplasm) to detect melanoma cells and Ki67 (Spring, sp-6, 1:100, nuclei) as a marker of proliferating nuclei.
Commercially available antibodies were used for the immunohistochemistry, with a control section for every batch of 20 sections, and evaluated blinded by a senior pathologist (TS). Ultraview fast red was used as detection system, to minimise the influence of the pigmentation of melanophages or melanoma cells. The sections were stained for cd163+ macrophages (Ab Serotec, EDHu-1, 1:100, cytoplasm) and cd66b+ granulocytes (BD Bioscience, G10F5, 1:200, cytoplasm). For tumour cell proliferation, we used double stains of MelanA (Cell Marque, M2-7C10, 1:50, cytoplasm) to detect melanoma cells and Ki67 (Spring, sp-6, 1:100, nuclei) as a marker of proliferating nuclei.
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