Lung digests were filtered through 100-μm strainers (Fisher), subjected to red-cell lysis (eBioscience), and stained with PE-labeled antimouse podoplanin (eBioscience) and APC-labeled antimouse LYVE-1 (R&D), with Aqua (Biolegend) viability marker for dead-cell exclusion. Dual PE/APC+ live cells were analyzed by an LSRII (BD) cytometer. The quantity of dual-positive cells as a percentage of total cells was analyzed/plotted and used in statistical analyses comparing lungs from mutant versus control mice.
Red cell lysis
Manufactured by Thermo Fisher Scientific
The Red-cell lysis product is a solution designed to lyse (break down) red blood cells, which is a common pre-treatment step in various analytical procedures. It functions by disrupting the cell membranes of red blood cells, allowing for the isolation and analysis of other cellular components.
Automatically generated - may contain errors
2 protocols using red cell lysis
1
Quantification of Podoplanin and LYVE-1 Expressing Cells
2
Quantitative Flow Cytometry Analysis of Lung Cells
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