Pyridine reagentplus

Manufactured by Merck Group

Sourced in Germany

Pyridine (ReagentPlus) is a clear, colorless liquid that is commonly used as a solvent and reagent in various laboratory applications. It has a distinctive, unpleasant odor. The product is intended for research and laboratory use only.

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Lab products found in correlation

Hplc grade chloroform, by Thermo Fisher Scientific (2 mentions) Lc ms grade water, by Thermo Fisher Scientific (2 mentions) Methylamine solution, by Merck Group (2 mentions)

Spelling variants of this product

Pyridine (718 citations) ReagentPlus (181 citations)

2 protocols using pyridine reagentplus

Lipid Standards for Mass Spectrometry

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We used 17:0/14:1 PC (LM1004), 17:0/14:1 PE (LM-1104), 17:0/14:1 PI (LM-1504), 17:0/14:1 PS (LM-1304), C17 ceramide (860517), and C8-glucosyl(β) ceramide (860540) as internal lipid standards, which were purchased from Avanti Polar Lipids (Alabaster, AL). Cholesterol was used as sterol standard and was purchased from Fluka. Pyridine (ReagentPlus) and methylamine solution (33% in absolute ethanol) were from Sigma-Aldrich. HPLC-grade chloroform was purchased from Acros (Geel, Belgium), and LC-MS–grade methanol and LC-MS–grade ammonium acetate were from Fluka. LC-MS–grade water was purchased from Fisher Scientific (Loughborough, United Kingdom).

da Silveira dos Santos A.X., Riezman I., Aguilera-Romero M.A., David F., Piccolis M., Loewith R., Schaad O, & Riezman H. (2014). Systematic lipidomic analysis of yeast protein kinase and phosphatase mutants reveals novel insights into regulation of lipid homeostasis. Molecular Biology of the Cell, 25(20), 3234-3246.

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Sphingolipid Extraction and Analysis

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Sphingolipid extraction and analysis were performed at University of Geneva, Geneva, Switzerland in the framework of collaborative work. Four, seven or eleven independent biological replicates were analyzed, each of which corresponding to 3 to 6 technical replicates. PC17:0/14:1 (LM1004), PE17:0/14:1 (LM-1104), PI17:0/14:1 (LM-1504), PS17:0/14:1 (LM-1304), C17:0-ceramide (860517), and C8-Glucosyl(β)ceramide (860540) were used as internal lipid standards and were purchased from Avanti Polar Lipids Inc. (Alabaster, AL, United States). Cholesterol was used as sterol standard and was purchased from Fluka (Sigma–Aldrich, Steinheim, Germany). Pyridine (ReagentPlus^®) and methylamine solution (33% in absolute ethanol) were from Sigma–Aldrich (Steinheim, Germany). HPLC-grade chloroform was purchased from Acros (Geel, Belgium), LC-MS grade methanol and LC-MS grade ammonium acetate were from Fluka (Sigma–Aldrich, Steinheim, Germany). LC-MS grade water was purchased from Fisher Scientific (Loughborough, United Kingdom).

Guerreiro J.F., Mira N.P., Santos A.X., Riezman H, & Sá-Correia I. (2017). Membrane Phosphoproteomics of Yeast Early Response to Acetic Acid: Role of Hrk1 Kinase and Lipid Biosynthetic Pathways, in Particular Sphingolipids. Frontiers in Microbiology, 8, 1302.

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