Rat pup USVs were recorded in a sound‐attenuated chamber using the Avisoft UltraSoundGate 116 microphone and Avisoft‐RECORDER USGH software (version 4.2; Avisoft Bioacoustics; Glienicke/Nordbahn, Germany, Avisoft‐RECORDER, RRID:SCR_014436) located in a temperature controlled room (22.5 ± 1.5°C). The microphone was affixed to the upper lid of the recording chamber, at a vertical distance of 20.5 cm from its floor. The recording chamber was constructed with black PVC boards (20 × 24 × 12 cm) and was lined with sound‐absorbing foam to insulate the recording environment from external noise. A heating pad (37°C) was placed on the floor of the chamber before the recordings to prevent temperature fluctuations of the animals. Pups were taken out individually from the cage in random order and placed in a 600 mL beaker that was lined on its bottom with paper towels to minimize scratching noises that would interfere with their USV signals in the recording. The pup inside the beaker was placed into the chamber for a 3‐min recording. The audio signals were sampled at 250 kHz and stored as WAV files. After the recording, the beaker was cleaned thoroughly with 70% ethanol in between every recording to minimize effects on their USV because of olfactory cues.
Ultrasoundgate 116 microphone
Manufactured by Avisoft
Sourced in Germany
The UltraSoundGate 116 is a high-quality microphone designed for capturing ultrasonic sounds. It features a flat frequency response and a bandwidth of up to 200 kHz, making it suitable for applications that require the recording of high-frequency signals.
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Lab products found in correlation
2 protocols using ultrasoundgate 116 microphone
1
Rat Pup Ultrasonic Vocalization Recording
2
Isolation-induced Ultrasonic Vocalizations in Pups
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Isolation-induced USVs were recorded from each pup on P6, P10, and P13 as previously described (Hofer et al., 2002) . Starting 2 hr into the dark cycle, dams were removed from the home cage and placed in a familiar transfer cage in a separate room. Home cages with litters were transferred to another holding room and placed on a heating pad. Starting after a 10 min (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint this version posted March 13, 2020. ; https://doi.org/10.1101/2020.03.12.989210 doi: bioRxiv preprint Maternal cannabis vapor exposure habituation period, individual pups were carried to a separate testing room and placed in the center of an empty 17" x 12" x 7.5" (L x W x H) standard rat cage with a 3x3 grid visible underneath.
USVs were recorded for 3 min by an UltraSoundGate 116 microphone (Avisoft Bioacoustics, Glienicke/Nordbahn, Germany) suspended 15 cm above the cage floor. Recordings from each session were analyzed offline using DeepSqueak software (Coffey et al., 2019) (link) to determine the number and types of calls. The number of grid crossings per session was determined from the video recordings and scored manually by a researcher who was blinded to treatment condition.
The copyright holder for this preprint this version posted March 13, 2020. ; https://doi.org/10.1101/2020.03.12.989210 doi: bioRxiv preprint Maternal cannabis vapor exposure habituation period, individual pups were carried to a separate testing room and placed in the center of an empty 17" x 12" x 7.5" (L x W x H) standard rat cage with a 3x3 grid visible underneath.
USVs were recorded for 3 min by an UltraSoundGate 116 microphone (Avisoft Bioacoustics, Glienicke/Nordbahn, Germany) suspended 15 cm above the cage floor. Recordings from each session were analyzed offline using DeepSqueak software (Coffey et al., 2019) (link) to determine the number and types of calls. The number of grid crossings per session was determined from the video recordings and scored manually by a researcher who was blinded to treatment condition.
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