Anti tnf pacificblue

Manufactured by BioLegend

Anti-TNF-PacificBlue is a product designed for use in laboratory settings. It functions as a fluorochrome-conjugated antibody that specifically binds to tumor necrosis factor (TNF). This product is intended for research use only and its core function is to facilitate the detection and analysis of TNF in various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

3 protocols using anti tnf pacificblue

HLA Tetramer Staining and Intracellular Cytokine Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

HLA tetramer staining of T cells was performed by incubation with 5 µg/ml tetramer diluted in tetramer staining buffer (2% FCS, 0.01% sodium azide, and 2 mM EDTA in PBS) for 30 min at 4°C. Afterwards, T cells were stained with CD8-PerCP (BioLegend; 301030) for 20 min at 4°C.
For ICS, 0.5–1 Mio cells/well were stimulated with individual peptides (10 µg/ml) in the presence of brefeldin A (Sigma-Aldrich; B7651), GolgiStop (BD Biosciences; 554724) and anti-CD107a-FITC monoclonal antibody (BD Biosciences; 555800) at 150 µl per well for 12–14 h. After incubation, cells were washed and stained with anti-CD8-PerCP (BioLegend; 301030) and anti-CD4-APC (BioLegend; 300518) antibodies followed by fixation and permeabilization for a further 20 min at 4°C (Cytofix/Cytoperm; BD Biosciences). After washing with permwash buffer, cytokines were stained with anti-TNF-PacificBlue (BioLegend; 502920) and anti-IFNγ-PE (BioLegend; 506507) antibodies for 20 min at 4°C. Flow cytometric measurements were performed on a FACSCanto II cytometer (BD Biosciences) and analyzed using FlowJo version 10.

Lübke M., Spalt S., Kowalewski D.J., Zimmermann C., Bauersfeld L., Nelde A., Bichmann L., Marcu A., Peper J.K., Kohlbacher O., Walz J.S., Le-Trilling V.T., Hengel H., Rammensee H.G., Stevanović S, & Halenius A. (2019). Identification of HCMV-derived T cell epitopes in seropositive individuals through viral deletion models. The Journal of Experimental Medicine, 217(3), jem.20191164.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis of T Cell Responses to TBEV

Check if the same lab product or an alternative is used in the 5 most similar protocols

T cell responses to TBEV were assessed using multi-color flow cytometry, and the monoclonal antibodies (mAbs) used were; anti-CD107a FITC, anti-CD4 Pacific Blue, anti-CD8 PerCP, anti-HLA-DR PerCP, anti-Ki67 FITC, anti-Ki67 Alexa Fluor 700, anti-Bcl2 PE, anti-CCR7 PE-Cy7, anti-MIP-1β PE, anti-CD14 BD horizon V500, anti-CD19 BD horizon V500, anti-perforin FITC and anti-granzyme B APC, anti-granzyme B PE-CF594 all from BD Biosciences (San Jose, CA). Anti-CD45RA APC-Cy7, anti TNF pacific blue, anti-IFN-γ Brilliant Violet 570, anti-CD27 Brilliant Violet 785, anti-CD27 Brilliant Violet 421, anti-Helios Pacific Blue, anti-T-bet Alexa Fluor 488, anti-T-bet PE-Cy7, anti-CCR7 Brilliant Violet 785, anti-CD279 Brilliant Violet 711, anti-CD27 biotin and anti-CD127 Brilliant Violet 570 were all from Biolegend (San Diego, CA). Anti-CD38 Alexa Fluor 700, anti-CD38 eFluor 650, anti-CD127 Alexa Fluor 780, anti-PD-1 (CD279) PE, anti-Eomes eFluor 660 and IgM eFluor 650 were all from eBioscience (San Diego, CA). Anti-CD4 Qdot 605, anti-CD8 Qdot705, anti-CD8 Qdot 605, Streptavidin-Qdot 585, anti-CD57 pure and Aqua Live/Dead were all from Invitrogen (Carlsbad, CA). Anti-CD3 ECD, anti-CD3 PE-Cy5, HLA-A2 CMV pp65 tetramer in PE and anti-CD56 ECD were from Beckman Coulter (Brea, CA). HLA-A2 ILLDNITTL tetramer in PE was kindly provided by the NIH Tetramer core facility.

Blom K., Braun M., Pakalniene J., Dailidyte L., Béziat V., Lampen M.H., Klingström J., Lagerqvist N., Kjerstadius T., Michaëlsson J., Lindquist L., Ljunggren H.G., Sandberg J.K., Mickiene A, & Gredmark-Russ S. (2015). Specificity and Dynamics of Effector and Memory CD8 T Cell Responses in Human Tick-Borne Encephalitis Virus Infection. PLoS Pathogens, 11(1), e1004622.

+ Open protocol

+ Expand

Multiparametric Analysis of T-cell Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols

ICS was performed as reported earlier [23 (link)]. Cells were stimulated with individual peptides or with an equal volume of water/ 10% DMSO in the presence of anti-CD107a (BD), GolgiStop (BD) and Brefeldin A (Sigma-Aldrich). After 12 h cells were stained for CD4-APC-Cy7 (BD), CD8-PECy7 (Beckman Coulter) and CD3-BV711 (Biolegend) and with Aqua Live Dead, fixed and permeabilized in (Cytoperm/Cytofix; BD) and further stained for IFNγ-Alexa Fluor 700 (BD Biosciences), anti-TNF-Pacific Blue (Biolegend), IL-10-PE and IL-2-APC (both BD).

Rammensee H.G., Wiesmüller K.H., Chandran P.A., Zelba H., Rusch E., Gouttefangeas C., Kowalewski D.J., Di Marco M., Haen S.P., Walz J.S., Gloria Y.C., Bödder J., Schertel J.M., Tunger A., Müller L., Kießler M., Wehner R., Schmitz M., Jakobi M., Schneiderhan-Marra N., Klein R., Laske K., Artzner K., Backert L., Schuster H., Schwenck J., Weber A.N., Pichler B.J., Kneilling M., la Fougère C., Forchhammer S., Metzler G., Bauer J., Weide B., Schippert W., Stevanović S, & Löffler M.W. (2019). A new synthetic toll-like receptor 1/2 ligand is an efficient adjuvant for peptide vaccination in a human volunteer. Journal for Immunotherapy of Cancer, 7, 307.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!