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Mouse and rabbit anti v5

Manufactured by Merck Group

Mouse and rabbit anti V5 are primary antibodies that recognize the V5 epitope tag. The V5 epitope is a short peptide sequence derived from the P and V proteins of the paramyxovirus of simian virus 5 (SV5). These antibodies can be used to detect and purify proteins that have been engineered to express the V5 tag.

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2 protocols using mouse and rabbit anti v5

1

Antibodies for BIN1 and Actin Cytoskeleton Analysis

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Rabbit polyclonal antibody (A#5299, Anaspec) was raised against BIN1 peptide LRKGPPVPPPPKHTPSKEMKQEQI (epitope encoded by exon 13). We also used antibodies directed against the exon 17 encoded MYC-binding domain of BIN1 (99D, Sigma), pan-BIN1 rabbit antibody against SH3 domain (Abcam), mouse and rabbit anti α-actinin (Abcam), rabbit anti Cav1.2 (Alomone), mouse and rabbit anti V5 (Sigma), rabbit anti actin (Sigma), rabbit anti GST (Santa Cruz Inc.).
LifeAct-mCherry was described previously47 (link). Bovine N-WASP was obtained from Addgene (Plasmid 33019: Pcs2-Bnwasp). HeLa and FT293 cells were cultured in DMEM under standard mammalian cell conditions. Lipofectamine 2000 (Invitrogen) was used for cDNA transfections in HeLa cells.
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2

Antibodies for BIN1 and Actin Cytoskeleton Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rabbit polyclonal antibody (A#5299, Anaspec) was raised against BIN1 peptide LRKGPPVPPPPKHTPSKEMKQEQI (epitope encoded by exon 13). We also used antibodies directed against the exon 17 encoded MYC-binding domain of BIN1 (99D, Sigma), pan-BIN1 rabbit antibody against SH3 domain (Abcam), mouse and rabbit anti α-actinin (Abcam), rabbit anti Cav1.2 (Alomone), mouse and rabbit anti V5 (Sigma), rabbit anti actin (Sigma), rabbit anti GST (Santa Cruz Inc.).
LifeAct-mCherry was described previously47 (link). Bovine N-WASP was obtained from Addgene (Plasmid 33019: Pcs2-Bnwasp). HeLa and FT293 cells were cultured in DMEM under standard mammalian cell conditions. Lipofectamine 2000 (Invitrogen) was used for cDNA transfections in HeLa cells.
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