[1-14C]-labelled fatty acids were purchased from Amersham Biosciences (Amersham, UK), and non-radioactive fatty acids and non-radioactive lipid standards from Larodan (Malmö, Sweden). Free CoA, bovine serum albumin (BSA), NADH and heptadecanoic acid methyl ester (17:0-Me) were supplied by Sigma-Aldrich (St. Louis, MO, USA). The [1-14C]-labelled acyl-CoAs were prepared according to the modified methods described by Sanchez et al. [32 (link)]. The other chemicals and solvents used for analysis were from Merck (Darmstadt, Germany) or Sigma-Aldrich.
Heptadecanoic acid methyl ester 17 0 me
Manufactured by Merck Group
Sourced in United States
Heptadecanoic acid methyl ester (17:0-Me) is a long-chain fatty acid methyl ester. It is commonly used as an internal standard in gas chromatography analysis.
Automatically generated - may contain errors
2 protocols using heptadecanoic acid methyl ester 17 0 me
1
Radiolabeled Fatty Acid Preparation
2
Preparation and Characterization of Radiolabeled Lipids
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[1-14 C]-labelled fatty acids were purchased from Perkin Elmer (Waltham, MA, USA), and non-radioactive fatty acids and sn-1-18:1-lysophosphatidylcholine were purchased from Larodan (Malmö, Sweden). Free CoA, bovine serum albumin (BSA) and heptadecanoic acid methyl ester (17:0-Me) were supplied by Sigma-Aldrich (St. Louis, MO, USA). The standards for thin-layer chromatography were obtained from Avanti Polar Lipids (Alabaster, AL, USA). The acyl-CoAs and [1-14 C]-labelled acyl-CoAs were prepared according to the modified method described by Sánchez et al. (1973) . The other biochemicals and solvents used for analysis were from Merck (Darmstadt, Germany) or Sigma-Aldrich.
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