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Legendplex cytokine bead array

Manufactured by BioLegend
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The LEGENDplex cytokine bead array is a multiplex assay that allows for the simultaneous detection and quantification of multiple analytes in a single sample. The core function of this product is to enable the measurement of various cytokines, chemokines, and other signaling proteins in biological samples.

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Lab products found in correlation

Cytoflex s cytometer, by Beckman Coulter (1 mentions) Zombie aqua viability stain, by BioLegend (1 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions) 0.4 μm polyester membrane, by Corning (1 mentions)

Close spelling variants of this product

LEGENDplex bead array Cytokine bead array Legendplex beads LEGENDplex

4 protocols using legendplex cytokine bead array

1

Cytokine Profiling of Intestinal LPLs

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106 freshly isolated LPLs isolated from intestinal mucosa were incubated overnight (18hrs) in complete RPMI with 10% FCS without additional stimulation. Supernatants were assessed for cytokine levels with the Biolegend LEGENDplex cytokine bead array following manufacturers instructions (Biolegend; San Diego CA). Cytokines assayed in this array included: IL-23, IL-5, IFNγ, TNF, IL-12 (p70), IL-4, IL-1β, GM-CSF, IL-9, IL-17F, IL-17A, IL-22 and IL-13.
Overstreet A.M., LaTorre D.L., Abernathy-Close L., Murphy S.F., Rhee L., Boger A.M., Adlaka K.R., Iverson A.M., Bakke D.S., Weber C.R, & Boone D.L. (2018). The JAK inhibitor ruxolitinib reduces inflammation in an ILC3-independent model of innate immune colitis. Mucosal immunology, 11(5), 1454-1465.
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2

Flow Cytometry and Cytokine Analysis

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Flow cytometry staining was performed using the antibodies in Table S2 in addition to the Zombie Aqua viability stain (BioLegend) and the Foxp3 Transcription Factor Staining Buffer Set (eBioscience). Flow cytometry was performed on a CytoFlex S cytometer (Beckman Coulter) with subsequent analysis using FlowJo (BD Biosciences).
Secretion of CCL5 and CXCL10 by dMMR and CIN CRCs into cell supernatants was analyzed using a custom LegendPlex cytokine bead array (BioLegend). Data were acquired on a CytoFlex S cytometer and analyzed in FlowJo.
Mowat C., Mosley S.R., Namdar A., Schiller D, & Baker K. (2021). Anti-tumor immunity in mismatch repair-deficient colorectal cancers requires type I IFN–driven CCL5 and CXCL10. The Journal of Experimental Medicine, 218(9), e20210108.
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3

ALI-Cultured Human Bronchial Epithelial Cells Infected with PR8 and IFN-γ Treatment

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Human bronchial epithelial cells (HBECs) were grown in air-liquid interface as described earlier (Jiang et al., 2018 (link)). Briefly, 0.2 mL of HBECs were seeded in type IV collagen-coated permeable transwell supports (6.5 mm or 24 mm, 0.4-μm polyester membrane, Corning, NY) at a density of 1×105 cells/ml. Then, 0.5 mL of fresh differentiation medium (Stem Cell Technologies, Vancouver, BC) was added to the lower chamber. After 48 h, medium was removed from the apical chamber and cells were maintained under ALI conditions for ten days. Then, HBECs were infected for 24 h with PR8 (2:1 MOI) and/or treated for 24 h with IFN-γ (20μg/mL). The levels of CCL2 were measured in supernatant using the LEGENDplex cytokine bead array (Biolegend, San Diego, CA) per manufacturer’s instructions.
Schmit T., Guo K., Tripathi J.K., Wang Z., McGregor B., Klomp M., Ambigapathy G., Mathur R., Hur J., Pichichero M., Kolls J, & Khan M.N. (2022). Interferon (IFN)-γ promotes monocyte-mediated lung injury during influenza infection. Cell reports, 38(9), 110456.
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4

Cytokine Profiling of Intestinal LPLs

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106 freshly isolated LPLs isolated from intestinal mucosa were incubated overnight (18hrs) in complete RPMI with 10% FCS without additional stimulation. Supernatants were assessed for cytokine levels with the Biolegend LEGENDplex cytokine bead array following manufacturers instructions (Biolegend; San Diego CA). Cytokines assayed in this array included: IL-23, IL-5, IFNγ, TNF, IL-12 (p70), IL-4, IL-1β, GM-CSF, IL-9, IL-17F, IL-17A, IL-22 and IL-13.
Overstreet A.M., LaTorre D.L., Abernathy-Close L., Murphy S.F., Rhee L., Boger A.M., Adlaka K.R., Iverson A.M., Bakke D.S., Weber C.R, & Boone D.L. (2018). The JAK inhibitor ruxolitinib reduces inflammation in an ILC3-independent model of innate immune colitis. Mucosal immunology, 11(5), 1454-1465.
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