Tcr β f1

Manufactured by Thermo Fisher Scientific

Sourced in Denmark, United States

The TCR β-F1 is a piece of lab equipment designed to detect and analyze T cell receptor (TCR) beta chain expression. It utilizes flow cytometry technology to quantify the presence and relative abundance of different TCR beta chain variants within a sample. The core function of the TCR β-F1 is to provide researchers with detailed information about the composition and diversity of the T cell repertoire in various biological samples.

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Lab products found in correlation

Clone ber h2, by Agilent Technologies (1 mentions)

2 protocols using tcr β f1

Immunohistochemical Analysis of Lymphoma Markers

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Immunohistochemistry was carried out with the following antibodies: CD30 (Monoclonal Mouse Anti-Human; Clone Ber-H2, 1:50, Dako, Denmark); TCR β-F1 (Monoclonal Mouse Anti-Human, Clone 8A3, 1:100, Thermo Scientific, IL 61105 USA); ALK1 mAb (1:100 kindly provided by Dr Karen Pulford, University of Oxford) as described in ref. 42 (link).

Malcolm T.I., Villarese P., Fairbairn C.J., Lamant L., Trinquand A., Hook C.E., Burke G.A., Brugières L., Hughes K., Payet D., Merkel O., Schiefer A.I., Ashankyty I., Mian S., Wasik M., Turner M., Kenner L., Asnafi V., Macintyre E, & Turner S.D. (2016). Anaplastic large cell lymphoma arises in thymocytes and requires transient TCR expression for thymic egress. Nature Communications, 7, 10087.

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Immunophenotyping of Extranodal NK/T-Cell Lymphoma

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Tissues from 148 cases of ENKTL, nasal type, diagnosed in the Samsung Medical Center from 1996 to 2011, were retrieved. Diagnosis was based on the 2008 WHO classification. 1 The histology of each case was reviewed. In all cases, immunohistochemistry was applied for CD3, CD30 (DAKO, Carpinteria, CA, USA), CD4 (Thermo Scientific, Waltham, MA, USA), CD5, CD8, CD56, granzyme B, and for EBV-encoded small RNAs using ISH kits for EBV (Leica Microsystems, Newcastle upon Tyne, UK). TCR gene rearrangement analyses and immunohistochemistry for TCR-βF1 and TCR-cγM1 (Thermo Scientific, Rockford, IL, USA) were performed for virtually all cases. Finally, 108 cases were selected for analysis. In addition, 4 μm sections of normal tonsil and appendix, two cases of enteropathy-associated T-cell lymphoma (EATL) of the TCR-αβ and TCR-γδ phenotypes, and one case of aggressive NK cell leukemia were used to compare the RNAscope assay results with those of immunohistochemistry.

Hong M., Lee T., Young Kang S., Kim S.J., Kim W, & Ko Y.H. (2016). Nasal-type NK/T-cell lymphomas are more frequently T rather than NK lineage based on T-cell receptor gene, RNA, and protein studies: lineage does not predict clinical behavior. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 29(5).

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