By4742

Manufactured by Horizon Discovery

BY4742 is a laboratory strain of the yeast Saccharomyces cerevisiae. It is a haploid strain derived from the S288C background and is commonly used in genetic and molecular biology research.

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By4741, by Horizon Discovery (1 mentions)

2 protocols using by4742

Yeast Strain Manipulation for Prion Research

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Yeast strains BY4742 (MAT his3∆1 leu2∆0 lys2∆0 ura3∆0) (obtained from GE Dharmacon) and 74-D-694 (MATa ade1-14 his3-200 ura3-52 leu2-3, 112 trp1-289) were used for the studies [58] . The other yeast strains used including ask10med13fis1 dnm1ybh3 mdv1caf4vps1andcnc1were all derivatives of BY4742 and obtained from the yeast MAT alpha knock out clone collection from GE Dharmacon [59, 60]  Isogenic [pin -] versions of the ask10med13fis1dnm1ybh3mdv1 caf4vps1andcnc1 yeast strains were obtained by growing the strains on YPD plates supplemented with 5 mM GuHCl for five passages as patches to eliminate any presence of the [PIN + ] prion. All studies were carried out in the [pin -] yeast strains [61] .

Bharathi V., Girdhar A, & Patel B.K. (2021). Role of CNC1 gene in TDP-43 aggregation-induced oxidative stress-mediated cell death in S. cerevisiae model of ALS. Biochimica et biophysica acta. Molecular cell research, 1868(6).

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Homologous Recombination for Yeast Strain Construction

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All strains from this study were made by homologous recombination as described (Longtine et al., 1998 (link); Janke et al., 2004 (link); Sheff and Thorn, 2004 (link)). BY4741 and BY4742 yeast strains were purchased from GE Dharmacon (Lafayette, CO). Strains were propagated in rich medium (YPD: 1% yeast extract, 2% peptone, 2% dextrose) or SD minimal medium (0.17% yeast nitrogen base, 0.5% ammonium sulfate, 2% synthetic complete mix, 2% dextrose) supplemented with the appropriate amino acids for plasmid selection. Plasmids were made by homologous recombination in yeast, rescued in Escherichia coli, and confirmed by sequencing. pHPH was made by PCR amplification of the hygromycin resistance gene HPH from pFA6a-hphNT1 and cotransformation of the PCR product with linearized pRS416. Proteins were tagged with either the bright GFP variant GFP+ (Scholz et al., 2000 (link)) or GFP(envy) (Slubowski et al., 2015 (link)), a gift from C. Slubowski (University of Massachusetts, Boston, MA), by PCR amplification of the GFP::HIS cassette and transformation into yeast. Strains and plasmids are described in Supplemental Tables S1 and S2.

Whitfield S.T., Burston H.E., Bean B.D., Raghuram N., Maldonado-Báez L., Davey M., Wendland B, & Conibear E. (2016). The alternate AP-1 adaptor subunit Apm2 interacts with the Mil1 regulatory protein and confers differential cargo sorting. Molecular Biology of the Cell, 27(3), 588-598.

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