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2 protocols using uv spectrophotometer uv 2600

1

Characterization of Silver Nanoparticles in Egg-White

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The AgNPs-EW were primarily characterized by UV-Vis absorption spectroscopy (Shimadzu UV spectrophotometer UV-2600). Fourier transform infrared spectroscopy (FTIR) was employed to characterize the interaction between the silver nanoparticles and protein present in the egg-white, and FTIR spectra were recorded in the range from 4000 to 400 cm−1 using a Spectrum RX-IFTIR (Perkin Elmer Massachusetts, USA). Lyophilized egg-white served as a control. Dynamic light scattering (DLS) analysis was carried out using a zeta sizer (Malvern Instrument Co. Ltd., UK) for evaluation of the AgNPs-EW stability. This analysis was performed using duplicate measurements and the size distribution on correlator software version Zetasizer 7.11. All the samples were diluted 100× with double distilled water before analysis.
Zeta potential measurements were carried out using a zeta sizer (Malvern Instrument Co. Ltd., UK) in a disposable cell at 25 °C. DLS and zeta potential measurements were performed to study the stability of AgNPs-EW. These measurements were carried out between 1 to 3 h after the preparation of the AgNPs-EW. The surface morphology and particles sizes of the AgNPs-EW were observed using high-resolution transmission electron microscopy (Hitachi H-7500).
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2

Herbicide and Adjuvant Protocol for Immunoassay

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Alachlor and 3-mercaptopropionic acid were purchased from Macklin (Shanghai, China). Pretilachlor, propisochlor, acetochlor, and metalaxyl were provided from TMRM (Beijing, China) and butachlor was purchased from Aladdin (Shanghai, China). PEG1450, Freund’s complete adjuvant (FCA), hypoxanthine-aminopterin-thymidine (HAT), Freund’s incomplete adjuvant (FIA), Ovalbumin (OVA), Bovine serum albumin (BSA), Peroxidase labeled goat anti-mouse immunoglobulin (HRP-IgG), and 1-Ethyl-3(3-dimethylaminopropyl) Carbodiimide (EDC) were obtained from Sigma (St. Louis, MO, USA), fetal bovine serum were provided from ExCell Bio (Beijing, China). Cell culture plates were obtained from NEST Biotechnology Co., Ltd. (Wuxi, China), N-hydroxysuccinimide (NHS), and other organic chemical reagents were provided from Sinopharm (Shanghai, China).
UV spectrophotometer (UV2600) and high-performance liquid chromatography (LC-20AD) from Shimadzu, Kyoto, Japan. The multifunctional enzyme labeling instrument was from PerkinElmer (Waltham, MA, USA).
All experimental animals used in this study were approved by the Animal Ethics Committee. Female Balb/C mice (6~8 weeks old) were provided by the Laboratory Animal Center of Huazhong Agricultural University.
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