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Bio plex pro human mmp panel 9 plex

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The Bio-Plex Pro Human MMP Panel 9-plex is a multiplex assay designed to measure the levels of 9 different matrix metalloproteinases (MMPs) in biological samples. This panel allows for the simultaneous quantification of MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-12, and MMP-13 using the Bio-Plex suspension array system.

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Lab products found in correlation

Bio plex 200 system, by Bio-Rad (2 mentions) Bio plex manager 6, by Bio-Rad (2 mentions) Bio plex pro human cytokine 27 plex assay, by Bio-Rad (2 mentions) Bio plex pro human cytokine 8 plex panel, by Bio-Rad (1 mentions) Bio plex manager software version 6, by Bio-Rad (1 mentions) Bio plex magpix system, by Bio-Rad (1 mentions) Bio plex pro human chemokine panel 40 plex, by Bio-Rad (1 mentions) Milliplex map kit, by Merck Group (1 mentions) Luminex xmap, by Merck Group (1 mentions)

Close spelling variants of this product

Bio-Plex Pro Human MMP panel Human Bio-Plex Pro Bio-Plex 9-Plex

6 protocols using bio plex pro human mmp panel 9 plex

1

Multiplex Cytokine and MMP Quantification

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Conditioned media samples with a total protein concentration of 800 μg/mL were used for the detection of secreted IL-2, IL-4, IL-6, IL-8, IL-10, GM-CSF, IFN-γ and TNF-α cytokines using a Bio-Plex Pro Human Cytokine 8-plex panel (Bio-Rad), as previously described10 (link). Secreted IL-1β was separately quantified using Bio-Plex Pro Human Cytokine Standard 27-plex, Group I, magnetic beads and detection antibodies for human IL-1β (Bio-Rad). MMP-1, -2, -3, -7, -8, -9, -10, -12 and 13 were assessed using a Bio-Plex Pro Human MMP panel 9-plex (Bio-Rad). The cytokine and MMPs levels were analysed according to the manufacturer’s instructions using a Bio-Plex MAGPIX System and Bio-Plex Manager software version 6.0 (Bio-Rad).
Geicu O.I., Stanca L., Voicu S.N., Dinischiotu A., Bilteanu L., Serban A.I, & Calu V. (2020). Dietary AGEs involvement in colonic inflammation and cancer: insights from an in vitro enterocyte model. Scientific Reports, 10, 2754.
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2

Multiplex Analysis of Chondrocyte MMP Expression

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Supernatants of micromasses derived from control siRNA (NC) or NOTCH1 siRNA (N1) chondrocytes were used to test the role of NOTCH1 on the expression of major matrix metalloproteinases, across micromass maturation.
To this end we used the Bio-Plex Pro™ Human MMP Panel, 9-Plex (Bio-Rad Laboratories, #171AM001M), a multiplex bead based sandwich immunoassay kit, with high sensitivity and dynamic range, to simultaneously evaluate the three collagenases (collagenase1/MMP-1, collagenase 2/MMP-8 and collagenase 3/MMP-13), the two stromelysins (stromelysin1/MMP-3 and stromelysin2/2MMP-10), the two gelatinase A (72 kDa, MMP-2) and B (92 kDa, MMP-9), the matrylisin/MMP-7, and the macrophage metalloelastase MMP-12.
Minguzzi M., Panichi V., D’Adamo S., Cetrullo S., Cattini L., Flamigni F., Mariani E, & Borzì R.M. (2021). Pleiotropic Roles of NOTCH1 Signaling in the Loss of Maturational Arrest of Human Osteoarthritic Chondrocytes. International Journal of Molecular Sciences, 22(21), 12012.
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3

Multiplex Measurement of Serum Chemokines and MMPs

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The levels of chemokines and cytokines were measured using Bio-Plex Pro Human Chemokine Panel 40-plex (Bio-Rad Laboratories, Hercules, CA, Cat # 171-AK99MR2). The concentrations of MMPs were measured using Bio-Plex Pro Human MMP Panel 9-plex (Bio-Rad Laboratories, Cat # 171-AM001 M). Based on expected chemokine or MMP values in serum and the known ranges for the Bio-Plex kits, serum samples were diluted for optimal detection. Samples were randomized for analysis using GraphPad Software (GraphPad Software, Inc, La Jolla, CA). For the 40-plex, 25 μl of each serum sample was diluted to 50 μl with provided serum diluent and then used for the immunoassay. For the 9-plex, 12.5 μl of each serum sample was diluted to 50 μl with the provided sample diluent HB. The manufacturer’s protocol was followed for the remainder of the protocol.
Greene A.D., Kendziorski J.A., Buckholz J.M., Niu L., Xie C., Pinney S.M, & Burns K.A. (2018). Elevated serum chemokines are independently associated with both endometriosis and uranium exposure. Reproductive toxicology (Elmsford, N.Y.), 84, 26-31.
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4

Multiplex Analysis of MMP, TIMP, and ERK Proteins

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MMP and TIMP protein concentrations were measured using multiplex, bead-based (Luminex) assays on a Bio-Plex 200 suspension array system in accordance with each manufacturer’s instructions. The Bio-Plex Pro Human MMP 9-Plex Panel and Bio-Plex Pro Human TIMP 4-Plex Panel (Bio-Rad Laboratories, Watford, UK) were used for detection of MMPs and TIMPs in cell medium. Total and Phospho ERK protein concentrations were measured in cell lysates using Milliplex MAP Kit (2-Plex Total/Phospho ERK, Merck KGaA, Darmstadt, Germany). Data were acquired on a validated and calibrated Bio-Plex 200 system (Bio-Rad Laboratories, Watford, UK) and analyzed with Bio-Plex Manager 6.0 software (Bio-Rad Laboratories, Watford, UK) with a detection target of 50 beads per region, low RP1 target for CAL2 calibration, and recommended doublet discriminator (DD) gates of 5000–25,000 for Bio-Plex. The median fluorescence intensity (MFI) was measured.
Kosowska A., Garczorz W., Kłych-Ratuszny A., Aghdam M.R., Kimsa-Furdzik M., Simka-Lampa K, & Francuz T. (2020). Sitagliptin Modulates the Response of Ovarian Cancer Cells to Chemotherapeutic Agents. International Journal of Molecular Sciences, 21(23), 8976.
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5

Multiplexed Quantification of Proteases and Cytokines

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Analysis of MMPs and TIMPs protein concentration was performed in cell medium using multiplex, bead-based (Luminex) assays on a Bio-Plex200 suspension array system according to each manufacturer’s instructions. Data were acquired on a validated and calibrated Bio-Plex 200 system (Bio-Rad Laboratories) and analyzed with Bio-Plex Manager 6.0 software (Bio-Rad Laboratories) with a detection target of 50 beads per region, low RP1 target for CAL2 calibration and recommended doublet discriminator gates of 5000–25,000 for Bio-Plex. The median fluorescence intensity was measured. Bio-Plex Pro Human MMP 9-Plex Panel (Bio-Rad Laboratories) was used for detection of MMPs. Bio-Plex Pro Human Cytokine 27-plex assay (Bio-Rad Laboratories) was used for detection of TNF-α and VEGF. Analysis of phosphoIκB (Ser32) and IκB was performed in cell medium using multiplex, bead-based (Luminex xMAP, Merck) as described earlier and according to manufacturer’s instructions.
Kosowska A., Gallego-Colon E., Garczorz W., Kłych-Ratuszny A., Aghdam M.R., Woz´niak M., Witek A., Wróblewska-Czech A., Cygal A., Wojnar J, & Francuz T. (2017). Exenatide modulates tumor–endothelial cell interactions in human ovarian cancer cells. Endocrine Connections, 6(8), 856-865.
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6

Profiling HGSOC Adhesion Factors

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Conditioned media was collected from HGSOC adhesion assays using two unique AAM donors and centrifuged at 1,000g for 15 minutes at 4°C. The supernatant was diluted 1:2 in 1% bovine serum albumin (BSA, Sigma, St. Louis, MO) in SFM and assayed by Bio-Plex Pro™ Human MMP 9-Plex Panel and Bio-Plex Pro™ Human Cytokine 27-plex Assay (Bio-Rad), using the MagPix® instrument (Luminex Corporation, Austin, TX).
Informed, written consent was obtained from patients recruited under a study approved by the Institutional Review Board at the University of Wisconsin-Madison. Studies were conducted in accordance with recognized ethical guidelines (e.g., Declaration of Helsinki, CIOMS, Belmont Report, U.S. Common Rule). Ascites was collected from patients with HGSOC (n=20) or benign conditions (n=4). Samples were diluted 1:2 in 1% BSA/PBS and assayed using a human MIP-1β DuoSet ELISA following manufacturer’s instructions.
Carroll M.J., Fogg K.C., Patel H.A., Krause H.B., Mancha A.S., Patankar M.S., Weisman P.S., Barroilhet L, & Kreeger P.K. (2018). Alternatively activated macrophages upregulate mesothelial expression of P-selectin to enhance adhesion of ovarian cancer cells. Cancer research, 78(13), 3560-3573.
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