Dynabeads his tag isolation and pulldown beads

Biotinylated human IgG, goat-anti-chicken DyLight®633 (GAC633), goat-anti-chicken DyLight®487 (GAC487), donkey-anti-rabbit DyLight®633 (DAC633), goat-anti-human IgG DyLight®633, and goat-anti-chicken HRP conjugate were purchased from Immunoreagents (Raleigh, NC). Chicken anti-c-myc antibody, rabbit-anti-HA antibody, streptavidin R-phycoerythrin conjugate (SA-PE), Dynabeads^™ Biotin Binder (streptavidin-coated beads), Dynabeads^™ His-Tag Isolation and Pulldown beads, High-fidelity Phusion^™ polymerase, and CloneJET^™ PCR cloning kit (K1231) were purchased from Thermo-Fisher (Waltham, MA). Mouse anti-penta-His antibody-Alexa Fluor 647 conjugate and Ni-NTA agarose were obtained from Qiagen (Valencia, CA). Frozen-EZ Yeast Transformation Kit^™, Zymoprep^™ Yeast plasmid Miniprep II kit, Quick-DNA^™ Miniprep Plus Kit were purchased from Zymo Research (Irvine, CA). All restriction enzymes were purchased from New England BioLabs (Ipswich, MA). Gene fragments were purchased from Integrated DNA Technologies (IDT; Coralville, IA). Oligonucleotide primers were purchased from IDT or Eton Biosciences (Raleigh, NC). Sequences of all primers and G-blocks are included in Table S1 (primers) and Table S2 (gene fragments). The composition of yeast culture media used was as previously described^{31 (link)}.