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α axin

Manufactured by Cell Signaling Technology
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α-Axin is a protein that plays a critical role in the Wnt signaling pathway, which is involved in various cellular processes such as cell proliferation, differentiation, and development. As a component of the β-catenin destruction complex, α-Axin helps regulate the stability and localization of β-catenin, a key transcriptional co-activator in the Wnt signaling pathway.

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Lab products found in correlation

α cd44, by Cell Signaling Technology (2 mentions) α hif 1a, by Cell Signaling Technology (2 mentions) α glut1, by Cell Signaling Technology (2 mentions) α vegfa, by Abcam (2 mentions) α β actin, by Abcam (2 mentions) α β catenin, by Cell Signaling Technology (2 mentions) α c myc, by Cell Signaling Technology (2 mentions) α e cadherin, by Cell Signaling Technology (2 mentions) α cyclin d1, by Cell Signaling Technology (2 mentions) α histone h3, by Cell Signaling Technology (2 mentions) Ne per nuclear and cytoplasmic extraction kit, by Thermo Fisher Scientific (2 mentions) Ripa buffer, by Beyotime (2 mentions)

2 protocols using α axin

1

Protein Expression Analysis in Breast Cancer

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The whole cell proteins of MDA-MB-231/MDA-MB-468 cells were extracted from cells using RIPA buffer (Beyotime, Shanghai, China) and the nuclear extracts were prepared with a NE-PER Nuclear and Cytoplasmic Extraction Kit (Pierce Biotechnology, Rockford, USA). Western blots were probed with α-phospho LRP6 (Ser1490), α-LRP6, α-β-catenin, α-E-cadherin, α-N-cadherin, α-Vimentin, α-Snail, α-Histone H3, α-c-Myc, α-cyclin D1, α-Axin, α-CD44, α-HIF-1a, α-Glut1 (Cell Signaling Technology, USA), α-VEGFA (Abcam, Cambridge, UK), α-β-actin.
Xie W., Zhao H., Wang F., Wang Y., He Y., Wang T., Zhang K., Yang H., Zhou Z., Shi H., Wang J, & Huang G. (2021). A novel humanized Frizzled-7-targeting antibody enhances antitumor effects of Bevacizumab against triple-negative breast cancer via blocking Wnt/β-catenin signaling pathway. Journal of Experimental & Clinical Cancer Research : CR, 40, 30.
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2

Western Blot Analysis of EMT Markers

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The whole cell proteins of MDA-MB-231/MDA-MB-468 cells were extracted from cells using RIPA buffer (Beyotime, Shanghai, China) and the nuclear extracts were prepared with a NE-PER Nuclear and Cytoplasmic Extraction Kit (Pierce Biotechnology, Rockford, USA). Western blots were probed with α-phospho LRP6 (Ser1490), α-LRP6, α-β-catenin, α-E-cadherin, α-N-cadherin, α-Vimentin, α-Snail, α-Histone H3, α-c-Myc, αcyclin D1, α-Axin, α-CD44, α-HIF-1a, α-Glut1 (Cell Signaling Technology, USA), α-VEGFA (Abcam, Cambridge, UK), α-β-actin.
Xie W., Zhao H., Wang F., Wang Y., He Y., Wang T., Zhang K., Yang H., Zhou Z., Shi H., Wang J., & Huang G. (2020). A novel humanized Frizzled-7-targeting antibody enhances antitumor effects of Bevacizumab against triple-negative breast cancer via blocking Wnt/β-catenin signaling pathway.
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