Nf κb p105 50

Brain tissues and thoracic aortas were harvested after the rats were sacrificed. The total proteins were extracted and separated via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Transferred onto nitrocellulose membranes and probed with the antibodies against: NF-κB p105/50 (1:1000, Abcam, Cambridge, MA, USA), EGFR (1:1000, Abcam, Cambridge, MA, USA). β-actin was taken as the loading control. The relative optical densities of the specific proteins were determined with Quantity One software (Bio-Rad Laboratories Inc., Hercules, CA, USA) and quantified using ImageJ software (NIH, USA).

PCNA, NFκB p105/50, NFκB p65 and phospho-NFκB p65 (Ser536) antisera were purchased from Cell Signaling Technology (Danvers, MA, USA); PSD95, GFAP, Ki67, MAP2, NFκB p105/50, NFκB p65 and GAPDH antisera were purchased from Abcam (Cambridge, MA, USA); c-Rel and actin antisera were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA); Synapsin-I, Homer-1 and NG2 antisera were purchased from Millipore (Billerica, MA, USA). Secondary antisera conjugated with fluorescent Alexa dye 488 and 647 and Cy3 were purchased from Invitrogen and Jackson Laboratories. HRP-conjugated secondary antibodies were purchased from Cell Signaling Technology or Invitrogen.