Rabbit polyclonal antibody against the C3d domain of human complement component 3 (C3), and Rabbit polyclonal antibody against the C4c domain of human complement component 4 (C4) were purchased from Dako. Mouse monoclonal anti human C5b-9 antibody directed against a neoepitope exposed on complement component 9 when incorporated into the terminal complement complex (TCC) is from BioPorto Diagnostics. Mouse monoclonal against human complement component 1q (C1q) is from Quidel. Rabbit polyclonal anti SA antibody is from Thermoscientific. Alexa fluor 488 goat anti rabbit IgG, Alexa fluor 594 goat anti mouse IgG and Alexa fluor 488 goat anti human from Molecular Probes. U0126, CRID-3, and AG1478 all used at concentration of (10 µM) from Tocris. Human purified C1q, Mannan-binding lectin (MBL), complement Factor B, C3, C6 proteins and sera depleted of C1q, C3, Factor B, C6 were from Quidel. The MBL deficient serum was obtained from an individual homozygous for the D (R52C, rs5030737) variant not able to activate the MBL dependent lectin pathway has been previously described (26 (link)). IdeS was purified as previously described (27 (link)) and was generously provided by Lars Björck and Inga-Maria Frick.
Abu-Humaidan A.H., Elvén M., Sonesson A., Garred P, & Sørensen O.E. (2018). Persistent Intracellular Staphylococcus aureus in Keratinocytes Lead to Activation of the Complement System with Subsequent Reduction in the Intracellular Bacterial Load. Frontiers in Immunology, 9, 396.
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