Synergy 4 hybrid plate reader

Manufactured by Agilent Technologies

Sourced in United States

The Synergy 4 Hybrid Plate Reader is a multi-mode microplate reader capable of absorbance, fluorescence, and luminescence detection. It is designed for a wide range of applications in life science research and drug discovery.

Automatically generated - may contain errors

Lab products found in correlation

Beta glow assay system, by Promega (2 mentions) Phire hot start 2 dna polymerase, by Thermo Fisher Scientific (1 mentions) Cerulenin, by Cayman Chemical (1 mentions)

Close spelling variants of this product

Synergy Hybrid plate reader Synergy 4 plate reader Synergy Hybrid Reader Synergy 4 Hybrid Hybrid Plate Reader Synergy plate reader Synergy 4 reader Synergy 4 plate Synergy 4

4 protocols using synergy 4 hybrid plate reader

Modified Yeast Two-Hybrid Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Modified yeast two-hybrid assays were performed as described (Bartel and Fields, 1997 ). Briefly, sygl-1 cDNA encoding wild-type, full-length SYGL-1 (aa 1-206), or full-length SYGL-1 carrying PIM mutations were cloned into the NcoI site in pACTII (Gal4 activation domain plasmid), generating pJK1580, pJK1581, pJK1582, pJK2094, pJK2095 and pJK2096 using the Gibson assembly method. The PUF repeat region of FBF-2 (aa 121-632) was cloned into the NdeI site in pBTMknDB (LexA-binding domain plasmid) to generate pJK2046. Activation and binding domain plasmids were co-transformed into the L40-ura strain using the Te-LiAc method (Gietz and Schiestl, 2007 (link)). lacZ reporter activity was assayed in defined media (SD) supplemented with -Leu-Trp using the Beta-Glow^® Assay System following commercially available protocols (Promega, E4720). In short, yeast cultures were grown to mid-log phase, diluted to the same optical density (0.1), and added to equal volumes of Beta-Glow^® reagent. Yeast clones were then incubated for 1 h at room temperature, and luminescence was quantitated using a Biotek Synergy 4 Hybrid plate reader and Gen5 software. A complete list of plasmids used in yeast two-hybrid assays is given in Table S3.

Ferdous A.S., Costa Dos Santos S.J., Kanzler C.R., Shin H., Carrick B.H., Crittenden S.L., Wickens M, & Kimble J. (2023). The in vivo functional significance of PUF hub partnerships in C. elegans germline stem cells. Development (Cambridge, England), 150(9), dev201705.

+ Open protocol

+ Expand

Modified Yeast Two-Hybrid Assay for SYGL-1 and FBF-2

Check if the same lab product or an alternative is used in the 5 most similar protocols

Modified yeast two-hybrid assays were performed as described (Bartel & Fields, 1997). Briefly, sygl-1 cDNA encoding wild-type full-length SYGL-1 (a.a. 1–206), or full-length SYGL-1 carrying PIM mutations were cloned into the Nco I site in pACTII (Gal4 activation domain plasmid), generating pJK1580, pJK1581, pJK1582, pJK2094, pJK2095, and pJK2096 respectively, using the Gibson assembly method. The PUF repeat region of FBF-2 (a.a. 121–632) was cloned into the Nde I site in pBTMknDB (LexA binding domain plasmid) to generate pJK2046. Activation and binding domain plasmids were co-transformed into the L40-ura strain using the Te-LiAc method (Gietz & Schiestl, 2007). LacZ reporter activity was assayed in defined media (SD) supplemented with -Leu-Trp using the Beta-Glow^® Assay System following commercially available protocols (Promega #E4720). In short, yeast cultures were grown to mid-log phase, diluted to the same optical density (0.1), and added to equal volumes of Beta-Glow^® reagent. Yeast clones were then incubated for 1 hr at room temperature, and luminescence was quantitated using a Biotek Synergy 4 Hybrid plate reader and Gen5 software (Winooski, VT). A complete list of plasmids used in yeast two-hybrid assays is available in Table S3.

Ferdous A.S., Costa Dos Santos S.J., Kanzler C.R., Shin H., Carrick B.H., Crittenden S.L., Wickens M, & Kimble J. (2023). Functional significance of PUF partnerships in C. elegans germline stem cells. bioRxiv.

+ Open protocol

+ Expand

Molecular Biology Techniques Verified

Check if the same lab product or an alternative is used in the 5 most similar protocols

All plasmids were verified by DNA sequencing. Purifications of all DNA were performed with kits from BioBasic. Synthetic oligonucleotides were purchased from IDT (Coralville, IA, USA). All plate reader assays were performed using a BioTek Hybrid Synergy 4 plate reader (Winooski, VT, USA). Restriction enzymes were purchased from New England Biolabs (Ipswich, MA, USA). Polymerase chain reactions were conducted using Phire Hot Start II DNA Polymerase from ThermoFisher Scientific (Waltham, MA, USA). Chemicals were purchased from Sigma Aldrich (St. Louis, MO, USA) and Alfa Aesar (Haverhill, MA, USA).

Lund S., Courtney T, & Williams G.J. (2019). Probing the substrate promiscuity of isopentenyl phosphate kinase as a platform for hemiterpene analogue production. Chembiochem : a European journal of chemical biology, 20(17), 2217-2221.

+ Open protocol

+ Expand

Biosynthesis of Malonyl-CoA Analogues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Materials and reagents were purchased from Sigma Aldrich (St. Louis, MO) unless otherwise noted. Isopropyl β-D-thioglactoside (IPTG) was purchased from Calbiochem (Gibbstown, NJ). Primers were purchased from Integrated DNA Technologies (Coralville, IA). E. cloni 10G electrocompetent cells were purchased from Lucigen Corporation (Middleton, WI). Cerulenin was purchased from Cayman Chemical (Ann Arbor, MI), and stocks were dissolved in DMSO. Commercial malonyl-CoA and methylmalonyl-CoA were purchased from CoALA Biosciences (Austin, TX). All cultures were grown in LB media with 100 μg/mL ampicillin unless otherwise stated. Absorbance and fluorescence readings were taken in clear flat-bottom and black flat-bottom 96-well plates (Greiner Bio-One), respectively, in a BioTek Hybrid Synergy 4 plate reader, unless otherwise stated. All Sanger sequencing was performed by Genewiz, Inc. (South Plainfield, NJ).

Kalkreuter E., Keeler A.M., Malico A.A., Bingham K.S., Gayen A.K, & Williams G.J. (2019). Development of a genetically-encoded biosensor for detection of polyketide synthase extender units in Escherichia coli. ACS synthetic biology, 8(6), 1391-1400.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!