Cd14 apc efluor 780

Cell suspensions were stained with combinations of the following fluorochrome-conjugated antibodies following established guidelines^{57 (link)}: CD56 PE/Dazzle 594 (1:200), CD137 PE/cyanine7 (1:50) and CD57 BV605 (1:25; all BioLegend); CD7 BV786 (1:25), CD56 BUV737 (1:50), CD3 BUV805 (1:50), streptavidin BUV395 (1:100), CD16 BUV496 (1:50) and CD337 (NKp30) BV421 (1:25; all BD Biosciences); CD159a (NKG2A) biotin/PE-Vio770 (both 1:50) and CD159c (NKG2C) PE (1:100; all REAfinity, Miltenyi Biotec); CD337 (NKp30) eFluor 450 (1:25), CD3 APC-eFluor 780 (1:50), CD14 APC-eFluor 780 (1:50) and CD19 APC-eFluor 780 (1:50; all Thermo Fisher) and anti-FcεRI, γ subunit-FITC (1:50; Merck). Dead cells were excluded using Fixable Viability Dye eFluor 780 (Thermo Fisher) or a Zombie Aqua Fixable Viability kit (BioLegend). Data were acquired on an LSR Fortessa (BD Biosciences). Cell sorting was performed on a FACSAria II (BD Biosciences). FlowJo v10 was used for analysis of flow cytometry data, and GraphPad Prism v8.4.3 was used for statistical analysis.