Third-instar larvae were dissected in ice-cold PBS and fixed in 4% paraformaldehyde for 30 min. The fixed tissues were stained following standard procedures. The primary antibodies used were: mouse anti-DLG, anti-Futsch and anti-β-tubulin antibodies from the Developmental Studies Hybridoma Bank; rabbit anti-DVGlut (Daniels et al., 2004 (link)), rabbit anti-GFP (A11122, Invitrogen) at 1:1000, rabbit anti-mCherry (632496, Clontech) at 1:1000, mouse anti-acetylated tubulin (T6793, Sigma) 1:1000, mouse anti-Tau (12-6400, Invitrogen) at 1:1000, Cy3-conjugated goat anti-HRP, and Cy5-conjugated goat anti-HRP. The following secondary antibodies (from Jackson ImmunoResearch) were used: Cy3-conjugated goat anti-rabbit IgG at 1:1000, Dylight-488-conjugated anti-mouse IgG at 1:1000, and Alexa-Fluor-647-conjugated goat anti-HRP at 1:1000.
Mouse anti tau 12 6400
Manufactured by Thermo Fisher Scientific
The Mouse anti-Tau (12-6400) is a laboratory reagent used for the detection and identification of Tau protein in biological samples. It is a monoclonal antibody that specifically binds to the Tau protein, which is a microtubule-associated protein found in neurons. This product can be used in various laboratory applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of Tau protein.
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Lab products found in correlation
Cy3 conjugated goat anti hrp, by Thermo Fisher Scientific (2 mentions)
Cy5 conjugated goat anti hrp, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 647 conjugated goat anti hrp, by Jackson ImmunoResearch (2 mentions)
Anti β tubulin, by Developmental Studies Hybridoma Bank (2 mentions)
Dylight 488 conjugated anti mouse igg, by Jackson ImmunoResearch (2 mentions)
Cy3 conjugated goat anti rabbit igg, by Jackson ImmunoResearch (2 mentions)
Mouse anti dlg, by Developmental Studies Hybridoma Bank (2 mentions)
Rabbit anti gfp a11122, by Thermo Fisher Scientific (1 mentions)
Mouse anti acetylated tubulin t6793, by Merck Group (1 mentions)
Rabbit anti mcherry, by Takara Bio (1 mentions)
Rabbit anti gfp, by Thermo Fisher Scientific (1 mentions)
Anti futsch, by Developmental Studies Hybridoma Bank (1 mentions)
Mouse anti acetylated tubulin, by Merck Group (1 mentions)
2 protocols using mouse anti tau 12 6400
1
Drosophila Larval Neurobiology Staining
2
Larval Nervous System Immunostaining
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Third instar larvae were dissected in ice-cold PBS and fixed in 4% PFA for 30 min. The fixed tissues were stained following standard procedures. The primary antibodies used were: mouse anti-DLG, anti-Futsch and anti-β-Tubulin antibodies from Developmental Studies Hybridoma Bank; rabbit anti-DVGlut (54) , rabbit anti-GFP (A11122, Invitrogen) at 1:1000, rabbit anti-mCherry (632496, Clontech) at 1:1000, mouse anti-Acetylated Tubulin (T6793, Sigma) 1:1,000, mouse anti-Tau (12-6400, Invitrogen) at 1: 1000, Cy3-conjugated goat anti-HRP, and Cy5conjugated goat anti-HRP. The following secondary antibodies (from Jackson ImmunoResearch ) were used: Cy3-conjugated goat anti-rabbit IgG at 1:1000, Dylight-488conjugated anti-mouse IgG at 1:1000, and Alexa-Fluor-647-conjugated goat anti-HRP at 1:1000.
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