Mononuclear cells were collected from the spleen and lumbar spinal cord as described previously (26 (link)–28 (link)). CD4+, CD11b+, and CD11c+ cells were isolated using the MACS system (Miltenyi Biotec, Bergisch Gladbach, Germany). Helper T cell differentiation was induced as described previously (5 (link), 28 (link), 31 (link)). For flow cytometric analysis, cells were stained with PerCP/Cy5.5 or BV421-conjugated anti-mouse CD4 rat monoclonal antibody (RM4-5; BD Biosciences, Franklin Lakes, NJ, USA). The cells were then fixed and permeabilized with Cytofix/Cytoperm reagent (BD Biosciences) and stained with PE-conjugated anti-mouse IFN-γ rat monoclonal antibody (XMG1.2; BD Biosciences) and APC- or PE-conjugated anti-mouse IL-17A rat monoclonal antibody (TC11-18H10; BD Biosciences). The samples were analyzed using a FACS Aria III system (BD Biosciences) and the FlowJo software (FlowJo, Ashland, OR, USA).
Pe conjugated anti mouse ifn γ rat monoclonal antibody xmg1.2
Manufactured by BD
The PE-conjugated anti-mouse IFN-γ rat monoclonal antibody (XMG1.2) is a laboratory reagent used to detect and quantify mouse interferon-gamma (IFN-γ) in various immunological applications. The antibody is labeled with the fluorescent dye R-Phycoerythrin (PE) for easy detection and visualization.
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Lab products found in correlation
Facs aria 3 system, by BD (2 mentions)
Cytofix cytoperm reagent, by BD (2 mentions)
Macs system, by Miltenyi Biotec (2 mentions)
Percp cy5, by BD (2 mentions)
Bv421 conjugated anti mouse cd4 rat monoclonal antibody rm4 5, by BD (2 mentions)
Apc or pe conjugated anti mouse il 17a rat monoclonal antibody tc11 18h10, by BD (2 mentions)
2 protocols using pe conjugated anti mouse ifn γ rat monoclonal antibody xmg1.2
1
Flow Cytometry Analysis of Helper T Cell Differentiation
2
Isolation and Flow Cytometry of Immune Cells
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Mononuclear cells were collected from the spleen and lumbar spinal cord as described previously 45, 46, 47 . CD4 + , CD11b + , and CD11c + cells were isolated using the MACS system (Miltenyi Biotec, Bergisch Gladbach, Germany). Helper T cell differentiation was induced as described previously 5, 32, 47 . For flow cytometric analysis, cells were stained with PerCP/Cy5.5 or BV421-conjugated anti-mouse CD4 rat monoclonal antibody (RM4-5; BD Biosciences, Franklin Lakes, NJ, USA). The cells were then fixed and permeabilized with Cytofix/Cytoperm reagent (BD Biosciences) and stained with PEconjugated anti-mouse IFN-γ rat monoclonal antibody (XMG1.2; BD Biosciences) and APC-or PE-conjugated anti-mouse IL-17A rat monoclonal antibody (TC11-18H10; BD Biosciences). The samples were analyzed using a FACS Aria III system (BD Biosciences) 14 and the FlowJo software (FlowJo, Ashland, OR, USA).
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